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Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems
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A pyrene-based fluorescent probe for H2S detection and cellular imaging.

Said Alam1, Xuanzuo Tao1, Yanxia Mao1

  • 1School of Environmental and Chemical Engineering, Jiangsu University of Science and Technology, Zhenjiang, 212000, China.

Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology
|March 2, 2025
PubMed
Summary

Researchers developed a new fluorescent probe, DPP, for sensitive and selective detection of hydrogen sulfide (H2S) in biological and environmental samples. This rapid detection method offers significant potential for disease research and monitoring.

Keywords:
Fluorescent probeH2S detectionHele cellPyreneSpectroscopy

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Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Chemical Biology

Background:

  • Hydrogen sulfide (H2S) is a crucial reactive sulfur species involved in numerous physiological and pathological processes.
  • Accurate detection of H2S in biological systems is vital for understanding its role in human diseases.

Purpose of the Study:

  • To synthesize and characterize a novel pyrene-based fluorescent probe, DPP, for selective and sensitive H2S detection.
  • To validate the probe's performance in biological and environmental samples.

Main Methods:

  • Synthesis of the pyrene-based fluorescent probe DPP.
  • Spectroscopic characterization using 1H NMR, FT-IR, UV, and fluorescence spectroscopy.
  • In vitro and in vivo assays using HeLa cells, water samples, and paper strip tests.

Main Results:

  • The DPP probe demonstrated high sensitivity with a detection limit of 0.63 µM.
  • The probe exhibited excellent selectivity for H2S against various interfering species.
  • Rapid detection of H2S in under 6 minutes was achieved, with successful application in cellular and environmental samples.

Conclusions:

  • The novel DPP probe is a sensitive, selective, and rapid tool for H2S detection.
  • DPP shows significant potential for monitoring H2S in biological systems and for environmental applications.