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Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time

Published on: August 26, 2009

Precision Self-assembly of 3D DNA Crystals Using Microfluidics.

Xugen Chen^1,2,3, Pan Fu^1,3, Karol Woloszyn⁴

¹Laboratory of Advanced Theranostic Materials and Technology, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo 315201, China.

Journal of the American Chemical Society

|March 3, 2025

Summary

Researchers developed a microfluidic method to create uniform, single DNA crystals. This breakthrough in DNA nanotechnology offers precise control over crystal size and quantity for advanced applications.

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Last Updated: May 6, 2026

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time

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Published on: August 26, 2009

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles

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Published on: May 8, 2015

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Published on: December 1, 2017

Area of Science:

DNA nanotechnology
Materials science
Microfluidics

Background:

Controlling size and quantity of 3D DNA crystals is crucial for applications.
Current methods face challenges in achieving uniformity and precise control.

Purpose of the Study:

To develop a novel strategy for synthesizing monodisperse 3D DNA single crystals.
To enable precise control over DNA crystal growth using microfluidic reactors.

Main Methods:

Utilized microfluidic double-emulsion droplets as nanoliter-scale microreactors.
Employed uniformly sized droplets that can adjust DNA concentration without leakage.
Leveraged confined volume to ensure single crystal formation per droplet.

Main Results:

Achieved synthesis of monodisperse 3D DNA single crystals with high success rate (up to 98.6% ± 0.9%).
Controlled crystal sizes ranging from 19.3 ± 0.9 μm to 56.8 ± 2.6 μm.
Demonstrated applicability to various DNA crystal types.

Conclusions:

The microfluidic droplet method provides precise control over DNA crystal growth.
This approach offers a new pathway for DNA crystal self-assembly and microengineering.
Enables scalable production of uniform DNA crystals for diverse applications.