Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

6.8K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
6.8K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

13.0K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
13.0K
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

4.6K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
4.6K
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

7.4K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
7.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Confidence-guided outlier refinement and collaborative embedding for unsupervised person re-identification.

Scientific reports·2026
Same author

DOCTER: a genetically encoded switchable protein module for ERα-mediated transcriptional regulation.

Gene therapy·2026
Same author

Effects of hydrostatic pressure on epithelial dome formation and stability.

Soft matter·2026
Same author

Self-supervised stain normalization empowers privacy-preserving and model generalization in digital pathology.

NPJ digital medicine·2025
Same author

POT, an optogenetics-based endogenous protein degradation system.

Communications biology·2025
Same author

Task-driven framework using large models for digital pathology.

Communications biology·2024

Related Experiment Video

Updated: May 24, 2025

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

25.1K

Recent Advances in Structured Illumination Microscopy: From Fundamental Principles to AI-Enhanced Imaging.

Heng Zhang1,2, Yunqi Zhu1,2, Luhong Jin1

  • 1Department of Biomedical Engineering, MOE Key Laboratory of Biomedical Engineering, Zhejiang Provincial Key Laboratory of Cardio-Cerebral Vascular Detection Technology and Medicinal Effectiveness Appraisal, Zhejiang University, Hangzhou, 310027, China.

Small Methods
|March 3, 2025
PubMed
Summary

Structured illumination microscopy (SIM) offers advanced biological imaging. This review details SIM principles, recent innovations in illumination and reconstruction, and the impact of deep learning (DL) for enhanced imaging and AI integration.

Keywords:
artificial intelligencedeep learninglive‐cell imagingstructured illumination microscopysuper‐resolution imaging

More Related Videos

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM
12:44

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM

Published on: September 29, 2014

19.9K
Quantitative Analysis of Autophagy using Advanced 3D Fluorescence Microscopy
09:59

Quantitative Analysis of Autophagy using Advanced 3D Fluorescence Microscopy

Published on: May 3, 2013

17.8K

Related Experiment Videos

Last Updated: May 24, 2025

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

25.1K
Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM
12:44

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM

Published on: September 29, 2014

19.9K
Quantitative Analysis of Autophagy using Advanced 3D Fluorescence Microscopy
09:59

Quantitative Analysis of Autophagy using Advanced 3D Fluorescence Microscopy

Published on: May 3, 2013

17.8K

Area of Science:

  • Microscopy and Imaging Technologies
  • Biophysics
  • Computational Biology

Background:

  • Structured illumination microscopy (SIM) is a key super-resolution technique.
  • Advances in optical methods and computational algorithms are crucial for biological imaging.
  • Deep learning (DL) shows potential in revolutionizing microscopy.

Purpose of the Study:

  • To review the fundamental principles of SIM.
  • To highlight recent advancements in SIM illumination, modulation, and reconstruction.
  • To explore the integration of DL and AI in SIM systems.

Main Methods:

  • Review of current literature on SIM techniques.
  • Analysis of DL applications in image enhancement and reconstruction for SIM.
  • Discussion of evaluation metrics for DL in SIM.

Main Results:

  • SIM technology has significantly advanced with new optical components and algorithms.
  • DL integration improves SIM image quality, speed, and reconstruction.
  • AI-driven smart microscopes are a future prospect.

Conclusions:

  • SIM is a powerful super-resolution tool with ongoing advancements.
  • DL and AI are poised to transform SIM imaging capabilities.
  • Future smart microscopes will leverage AI for enhanced biological insights.