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Biofilms

Biofilms

Biofilms are complex communities of microorganisms encased in a self-produced extracellular polysaccharide matrix attached to surfaces. These microbial consortia can include single or multiple species, providing enhanced survival benefits by forming organized, multilayered structures.The formation of biofilms occurs through four key stages: attachment, colonization, development, and dispersal.During attachment, free-swimming planktonic cells adhere to a surface, often facilitated by...

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A Platform of Anti-biofilm Assays Suited to the Exploration of Natural Compound Libraries

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Decoding interactions between biofilms and DNA nanoparticles.

Alexandra Sousa¹, Rutuparna Kulkarni^2,3, Mona Johannessen⁴

¹Drug Transport and Delivery Research Group, Department of Pharmacy, UIT The Arctic University of Norway, Tromsø, Norway.

|March 3, 2025

Summary

This summary is machine-generated.

DNA-based nanoparticles show promise for combating biofilm infections. Chitosan-coated nanostructures demonstrated enhanced biofilm retention, suggesting potential for targeted drug delivery in antimicrobial therapy.

Keywords:

Chitosan DNA nanoparticles Diffusion coefficient Microfluidics Nucleic acid P. aeruginosa biofilms

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Published on: March 3, 2023

Area of Science:

Biomaterials Science
Nanotechnology
Microbiology

Background:

Biofilms pose significant challenges in antimicrobial therapy due to inherent tolerance to conventional antibiotics.
Advanced drug delivery systems are crucial for effective biofilm-associated infection treatment.
DNA-based nanoparticles (NPs) are an underexplored resource for antibiofilm strategies.

Purpose of the Study:

To investigate the diffusion, penetration, and retention of DNA-based nanocarriers within *P. aeruginosa* biofilms.
To evaluate the impact of nanocarrier design and modification on their behavior within biofilms.
To lay the groundwork for the therapeutic exploitation of DNA-based NPs in antibiofilm therapy.

Main Methods:

Formation of plain and modified DNA-based NPs using Watson-Crick base pairing, hydrophobic interactions, or electrostatic interactions via microfluidic mixing.
Assessment of nanocarrier interactions with biofilm structures using Single Plane Illumination Microscopy - Fluorescence Correlation Spectroscopy (SPIM-FCS) and Confocal Laser Scanning Microscopy (CLSM).
Analysis of the influence of microfluidic parameters on physicochemical properties and biofilm behavior of modified DNA NPs.

Main Results:

Single stranded DNA micelles (ssDNA micelle) and tetrahedral DNA nanostructures (TDN) exhibited similar diffusion and penetration profiles in *P. aeruginosa* biofilms.
Chitosan-coated TDN (TDN-Chit) displayed reduced diffusion and increased retention within the biofilm.
The enhanced retention of TDN-Chit is attributed to its larger size and positive surface charge.

Conclusions:

DNA can serve as a versatile building block for developing drug delivery systems targeting biofilms.
The behavior of DNA-based NPs within biofilms is influenced by their physicochemical properties, such as size and surface charge.
These findings are pivotal for selecting appropriate therapeutic agents for encapsulation and optimizing nanocarrier systems for biofilm-related infections.