Characterizing the microbial community constructure and the metabolites among different colour Moutai Daqu

There are three types of Daqu produced during the fermentation of Moutai Daqu, which are named as white, yellow and black Daqu. However, in-depth studies for them are lacking. Herein, the high-throughput sequencing and metabolomics techniques were used to analyze the differences in Moutai Daqu. The findings indicated that the predominant microorganisms in yellow and white Daqu were Kroppenstedtia and Bacillus, while Oceanbacillus and Scopulibacillus emerged as the primary microorganisms in black Daqu. Further exploration revealed that white and black Daqu played important roles in the liquefaction and saccharification processes. Besides, the results of metabolomics reveals that yellow and black Daqu exhibit a higher abundance of up-regulated amino acids and fatty acids, which exert a more significant effect on Moutai Baijiu flavor and bioactivity. This study reveals the differences among the three types of Moutai Daqu through comprehensive analysis, which provides technical support for improving the quality of Moutai Daqu.