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Sprouting and network data assessed in the chicken aortic ring assay under stimulation with human adipose-derived stem cell secretomes harvested from single cells or spheroids of different sizes

  • 0Division of Surgical Research, University Hospital of Zurich, 8091 Zurich, Switzerland.
Data in Brief +

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March 3, 2025

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March 3, 2025

View abstract on PubMed

Summary

This summary is machine-generated.

This study standardizes the chicken aortic ring assay for assessing drug-induced angiogenesis. It presents comprehensive readouts using human adipose-derived stem cell secretomes from various cultures to improve result comparability.

Area Of Science

  • Biomedical Engineering
  • Cell Biology
  • Pharmacology

Background

  • The chicken aortic ring assay is a standard method for evaluating drug effects on angiogenesis.
  • Current research lacks standardized terminology and overlapping parameters, hindering direct comparison of results.
  • This variability complicates the assessment of angiogenic modulation by different compounds.

Purpose Of The Study

  • To address the lack of standardization in the chicken aortic ring assay.
  • To present a comprehensive set of readouts for the assay.
  • To facilitate direct comparison of angiogenic modulation studies.

Main Methods

  • Utilized the chicken aortic ring assay to assess angiogenic modulation.
  • Employed secretomes from human adipose-derived stem cells (hADSCs) as a stimulant.
  • Cultivated hADSCs as single-cell monolayer cultures and 3D spheroids (250 and 8000 cells/spheroid).

Main Results

  • Collected data covering the full spectrum of possible readouts in the aortic ring assay.
  • Demonstrated the assay's response to hADSC secretomes from different culture conditions.
  • Provided a basis for comparing results across studies using standardized parameters.

Conclusions

  • The presented data establish a comprehensive framework for the chicken aortic ring assay.
  • Standardized readouts will enhance the reliability and comparability of angiogenesis research.
  • This approach supports more accurate evaluation of angiogenic modulators.

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