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Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
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Updated: May 24, 2025

Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation

Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation

Published on: April 19, 2019

A Novel Gene Stacking Method in Plant Transformation Utilizing Split Selectable Markers.

Guoliang Yuan¹, Md Torikul Islam^2,3, Gerald A Tuskan^2,3

¹Energy and Environment Directorate, Pacific Northwest National Laboratory, Richland, WA, USA.

|March 3, 2025

Summary

This summary is machine-generated.

We developed a novel split selectable marker system for plant gene stacking, simplifying the selection of multiple gene introductions using a single antibiotic. This intein-mediated method accelerates genetic improvement in various plant species.

Keywords:

Arabidopsis Intein Plant transformation Poplar Protein splicing Selectable marker

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Last Updated: May 24, 2025

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Published on: January 11, 2011

Area of Science:

Plant biotechnology
Molecular biology
Genetics

Background:

Gene stacking is crucial for enhancing plant traits through genetic modification.
Traditional co-transformation often requires multiple selectable marker genes, complicating the selection process.
Optimizing selection with multiple antibiotics for plant transformation can be challenging.

Purpose of the Study:

To develop an efficient and simplified method for gene stacking in plants.
To overcome the limitations of using multiple selectable marker genes in plant co-transformation.
To facilitate the introduction of multiple genes into both herbaceous and woody plants.

Main Methods:

Developed an intein-mediated split selectable marker system using two binary vectors.
Each vector contained a subset of genes of interest and a partial selectable marker gene fragment.
Utilized Agrobacterium-mediated co-transformation and selection with a single antibiotic (e.g., kanamycin).

Main Results:

Successfully demonstrated the use of a single selectable marker gene for co-transformation.
The split-marker system enabled efficient gene stacking in plants.
The method proved effective for both herbaceous and woody plant species.

Conclusions:

The intein-mediated split selectable marker system simplifies plant co-transformation and gene stacking.
This approach accelerates the genetic improvement of plants for polygenic or multiple traits.
The system enhances the scalability and feasibility of plant genetic engineering.