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Updated: May 24, 2025

Visualization of Endoplasmic Reticulum Subdomains in Cultured Cells
Published on: February 18, 2014
Dynamic visualization of endoplasmic reticulum viscosity in living cells with a multirotor-based fluorescent probe
Yuehua Zhang1,2, Nuo Dong1,2, Shiyu Nan1,2
1Key Laboratory of Medicinal Chemistry and Molecular Diagnosis (Ministry of Education), Key Laboratory of Chemical Biology of Hebei Province, Hebei University, Baoding, 071002, P. R. China. zhangpingzhu18@163.com.
Abstract:
Changes in viscosity can significantly influence the functionality of the endoplasmic reticulum (ER), and viscosity-responsive fluorescent probes can provide valuable feedback on its physiological state. In this study, three multirotor-based fluorescent probes (1-3) were designed and synthesized. Probes 1 and 2 exhibited remarkable viscosity sensitivity and specific targeting ability towards the ER. Probe 1 was utilized for in situ dynamic visualization of ER viscosity changes during inflammatory responses, drug treatments, and reticulophagy.

