Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Proteomics01:33

Proteomics

7.2K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
7.2K
Ribosome Profiling02:24

Ribosome Profiling

3.4K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Imaging-Guided Omics Technologies for Resolving Rare Cancer States and Advancing Nanomedicine.

Nano letters·2026
Same author

Unraveling the Molecular Mechanisms Underlying Spontaneous Multipolar Mitosis Through CIN-seq.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Plasmonic Enhancement of Fluorescence and Protein Dynamics in Living Mammalian Cells.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Investigating the Metabolic Heterogeneity of Cancer Cells Using Functional Single-Cell Selection and nLC Combined with Multinozzle Emitter Mass Spectrometry.

Analytical chemistry·2023
Same author

Genomic Exploration of Distinct Molecular Phenotypes Steering Temozolomide Resistance Development in Patient-Derived Glioblastoma Cells.

International journal of molecular sciences·2023
Same author

Instant processing of large-scale image data with FACT, a real-time cell segmentation and tracking algorithm.

Cell reports methods·2023
Same journal

Isolation of Mesenchymal Stem Cell-Derived Extracellular Vesicles.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Modeling Melanoma Immune Surveillance by CAR-T Cells in Human Skin Organoids.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Stepwise Optimization of a Matrigel-Based In Vitro Angiogenesis Assay for Reproducible and Quantifiable 2D-Tube Formation Using HUVECs.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Quantifying Mechanical Properties of Fresh Ovarian Tissue with Optical Brillouin Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

3D Chromatin Architecture During Early Development: New Methods and New Findings.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Metabolic Plasticity in Embryogenesis Throughout the Lens of NAD<sup></sup>.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: May 24, 2025

Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier
06:13

Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier

Published on: December 9, 2022

3.8K

Functional Single-Cell Proteomics: Technology and Biological Applications.

Pin-Rui Su1,2,3, Miao-Ping Chien4,5

  • 1Department of Chemistry, National Taiwan University, Taipei, Taiwan.

Methods in Molecular Biology (Clifton, N.J.)
|March 3, 2025
PubMed
Summary
This summary is machine-generated.

We developed FUNpro, a new microscopy technique for single-cell proteomics. This method allows researchers to identify and isolate specific cells based on their dynamic phenotypes for deeper proteomic analysis.

Keywords:
MicroscopyPhenotype-to-proteome linkingPhotolabelingSCoPE-MSSingle-cell proteomics

More Related Videos

Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells
07:55

Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells

Published on: June 21, 2019

5.7K
Single-Cell Factor Localization on Chromatin using Ultra-Low Input Cleavage Under Targets and Release using Nuclease
09:20

Single-Cell Factor Localization on Chromatin using Ultra-Low Input Cleavage Under Targets and Release using Nuclease

Published on: February 1, 2022

2.6K

Related Experiment Videos

Last Updated: May 24, 2025

Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier
06:13

Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier

Published on: December 9, 2022

3.8K
Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells
07:55

Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells

Published on: June 21, 2019

5.7K
Single-Cell Factor Localization on Chromatin using Ultra-Low Input Cleavage Under Targets and Release using Nuclease
09:20

Single-Cell Factor Localization on Chromatin using Ultra-Low Input Cleavage Under Targets and Release using Nuclease

Published on: February 1, 2022

2.6K

Area of Science:

  • Proteomics
  • Cell Biology
  • Cancer Research

Background:

  • Single-cell proteomics is crucial for understanding tumor heterogeneity.
  • Existing methods like SCoPE-MS profile many proteins but have limitations in functional screening.
  • Linking cellular proteomes to specific phenotypes remains a challenge.

Purpose of the Study:

  • To introduce FUNpro, a novel microscopy-based functional single-cell proteomic profiling technology.
  • To enable high-throughput, real-time live-cell screening, identification, and isolation of single cells.
  • To provide a generalized protocol for applying FUNpro to cultured cell lines, including cancer cells.

Main Methods:

  • Development of a microscopy-based platform for functional single-cell proteomic profiling (FUNpro).
  • Integration of live-cell imaging, photolabeling, fluorescence-activated cell sorting (FACS), and single-cell proteomics.
  • Application to a standard cancer cell line for protocol validation.

Main Results:

  • FUNpro enables high-throughput, real-time screening and isolation of single cells based on dynamic or rare phenotypes.
  • The technology successfully links proteomes of individual cells with their observed phenotypes.
  • A generalized protocol for microscopy, cell culture, image analysis, and proteomics is outlined.

Conclusions:

  • FUNpro offers a versatile solution for functional single-cell proteomic profiling in cultured cells.
  • This technology advances the ability to study cellular heterogeneity and identify cells of interest.
  • The described protocol facilitates the application of FUNpro in various research settings, including cancer research.