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    Super-resolution ultrasound (SRUS) imaging, or ultrasound localization microscopy (ULM), achieves high-resolution deep tissue visualization in vivo. This review details ULM

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    Area of Science:

    • Medical Imaging
    • Biophysics
    • Ultrasound Technology

    Background:

    • Ultrasound localization microscopy (ULM) enables super-resolution ultrasound (SRUS) imaging.
    • ULM visualizes deep tissues in vivo with unprecedented resolution using microbubbles (MBs).

    Purpose of the Study:

    • To review the key technical steps involved in ULM.
    • To provide perspectives on current techniques and future directions in ULM.

    Main Methods:

    • Focus on data acquisition, tissue clutter removal, and motion correction.
    • Detail localization, tracking, and image visualization techniques.
    • Review methods for evaluating ULM image quality.

    Main Results:

    • SRUS imaging via ULM offers superior resolution for deep tissue visualization.
    • Comprehensive review of state-of-the-art ULM techniques and influencing factors.

    Conclusions:

    • ULM is a powerful technique for high-resolution in vivo imaging.
    • Understanding ULM's technical parameters is crucial for optimal image quality and application.