Physalis peruviana heteropolysaccharide-conjugated selenium nanoparticles: Preparation, characterization, and...

Selenium nanoparticles (SeNPs) have attracted considerable attention in the field of cancer therapy due to their remarkable biological activities and advantageous nanoscale properties. However, their inherent instability presents a considerable challenge for broader applications. To address this issue, the heteropolysaccharide extracted from golden berries (the fruits of Physalis peruviana), designated as DLG, was utilized to synthesize heteropolysaccharide-conjugated selenium nanoparticles, named DLG-SeNP3. Subsequent characterization showed that the nanoparticles DLG-SeNP3 were spherical, with an average particle size of 77 nm, a zeta potential of -14.4 mV, and excellent stability under physiological pH conditions. Further biological investigations showed that DLG-SeNP3 effectively inhibited tumor cell proliferation, exhibiting an IC₅₀ value of 41.60 μg/mL against A549 cells, and induced apoptosis, with a proportion of 31.30 % at 100 μg/mL. Moreover, in vivo experiments demonstrated that DLG-SeNP3 not only inhibited angiogenesis, resulting in a 26.51 % decrease at 2 μg/mL, but also suppressed tumor growth and invasion, with reductions of 76.90 % and 66.67 % in the intensity and foci of red fluorescence, respectively, at 2 μg/mL. In conclusion, DLG-SeNP3, stabilized with polysaccharides derived from golden berries, shows promising potential for application in cancer treatment.