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Related Experiment Video

Updated: May 24, 2025

A Rapid and Chemical-free Hemoglobin Assay with Photothermal Angular Light Scattering

A Rapid and Chemical-free Hemoglobin Assay with Photothermal Angular Light Scattering

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Hemoglobin Mass Determination: Exploring Carboxyhemoglobin Stability and Analyzer Interchangeability.

Janne Bouten^1,2, Johan Garcia^1,3, Romain Carin^4,5

¹Laboratory Sport, Expertise and Performance (EA 7370), French Institute of Sport (INSEP), Paris, France.

Drug Testing and Analysis

|March 3, 2025

Summary

This summary is machine-generated.

Carbon monoxide (CO) rebreathing is key for measuring hemoglobin mass (Hb_mass). This study found that carboxyhemoglobin (HbCO) measurements are stable and interchangeable between analyzers, even with delayed analysis, supporting its use in research.

Keywords:

HbCO carbon monoxide rebreathing red blood cell count reliability

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Area of Science:

Sports Medicine
Physiology
Analytical Chemistry

Background:

Carbon monoxide (CO) rebreathing is a standard method for determining hemoglobin mass (Hb_mass).
Accurate carboxyhemoglobin (HbCO) measurement is essential for reliable Hb_mass determination in research settings, particularly during hypoxic or heat training and high-altitude studies.

Purpose of the Study:

To investigate the stability of HbCO levels.
To assess the interchangeability of two different Radiometer analyzer models (ABL 90 and ABL 825) for HbCO determination.
To evaluate the impact of delayed blood sample analysis on Hb_mass calculations.

Main Methods:

Twelve subjects underwent a CO rebreathing test.
Capillary blood samples were analyzed immediately on Day 1 using an ABL 90 analyzer.
Additional samples were stored and analyzed 4-8 days later using an ABL 825 analyzer.

Main Results:

A trivial mean difference was observed for both ΔHbCO (0.05%) and Hb_mass (7.7 g) between immediate and delayed analysis.
High reliability (ICC > 0.98) and low typical error (< 0.91%) were found for both ΔHbCO and Hb_mass.
The paired sample t-test indicated no significant differences between the two measurement conditions.

Conclusions:

Delayed analysis of HbCO samples, even with a different analyzer, provides reliable and interchangeable results for Hb_mass determination.
While immediate analysis with the same analyzer is still recommended, delayed analysis is a viable alternative when logistical constraints exist.
This finding supports the flexibility of CO rebreathing protocols in diverse research environments.