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Related Concept Videos

Sputum Studies II: Culture and Sensitivity01:20

Sputum Studies II: Culture and Sensitivity

Description
Sputum culture and sensitivity is a medical procedure used to diagnose bacterial infections in the respiratory tract and select the most appropriate antibiotics for treatment. This process involves analyzing sputum samples of thick and opaque secretions produced in the lungs and airways. These samples are collected from patients and then sent to the laboratory for analysis.
The test can identify various pathogens responsible for respiratory infections, including Streptococcus,...

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Related Experiment Video

Updated: May 8, 2026

Design of Cecal Ligation and Puncture and Intranasal Infection Dual Model of Sepsis-Induced Immunosuppression
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Design of Cecal Ligation and Puncture and Intranasal Infection Dual Model of Sepsis-Induced Immunosuppression

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An international observational study validating gene-expression sepsis immune subgroups.

David B Antcliffe1, Estelle Peronnet2,3, Frédéric Pène4

  • 1Division of Anaesthetics, Pain Medicine and Intensive Care, Imperial College London, London, UK.

Critical Care (London, England)
|March 3, 2025
PubMed
Summary
This summary is machine-generated.

This study developed a rapid RNA test to identify sepsis patients at high risk of poor outcomes. The gene-expression diagnostic tool shows potential for guiding precision medicine in critical care settings.

Keywords:
Gene-expressionProspective studySepsisTranscriptomics

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A Data-Driven Approach to Quantifying Immune States in Sepsis
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A Data-Driven Approach to Quantifying Immune States in Sepsis
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A Data-Driven Approach to Quantifying Immune States in Sepsis

Published on: February 7, 2025

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Area of Science:

  • Critical Care Medicine
  • Genomics
  • Translational Research

Background:

  • Sepsis sub-phenotypes with prognostic value have been identified retrospectively.
  • Current methods lack bedside applicability for rapid risk stratification.
  • There is a need for actionable diagnostics to guide sepsis management.

Purpose of the Study:

  • To identify high-risk sepsis patient subgroups using a rapid, multiplex RNA-based test.
  • To translate retrospective gene-expression findings into a clinically deployable tool.
  • To assess the prognostic capability of gene-expression profiles in intensive care unit (ICU) patients.

Main Methods:

  • Adult sepsis patients were recruited from 17 UK, Swedish, and French ICU sites.
  • Blood samples were collected at three distinct time points (S1, S2, S3) post-ICU admission.
  • Two risk models, based on the Immune-Profiling Panel prototype, were used for patient classification.

Main Results:

  • 357 patients were recruited with a 90-day mortality rate of 30%.
  • High-risk patient proportions decreased over sampling times (Model 1: 53% to 15%; Model 2: 81% to 37%).
  • Model 1 showed significantly higher 90-day mortality in high-risk groups at all time points (p<0.05).

Conclusions:

  • Gene-expression diagnostics can effectively identify sepsis patients at high risk of adverse outcomes.
  • This RNA-based approach holds promise for enabling precision medicine trials in sepsis.
  • The developed test offers a potential bedside tool for improved sepsis patient management.