Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA-seq03:21

RNA-seq

9.8K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.8K
Ribosome Profiling02:24

Ribosome Profiling

3.4K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.4K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

2.8K
2.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Vertebrate Genomes Project Phase I: A global reference genome resource.

bioRxiv : the preprint server for biology·2026
Same author

Incidental Renal Cell Carcinoma in an Active-Duty Fighter Pilot.

Aerospace medicine and human performance·2026
Same author

Recognising intoxication in healthcare: evidence, challenges, and implications.

Addiction science & clinical practice·2026
Same author

The effect of Montmorency tart cherry consumption on athletic performance and post-exercise recovery in healthy adults: a scoping review.

Frontiers in nutrition·2026
Same author

Higher disease burden and greater small fibre impairment in women with painful diabetic neuropathy.

Pain reports·2026
Same author

Percutaneous Image-Guided Non-Target Renal Biopsy in Cancer Patients: A Tertiary Cancer Center Experience.

Current oncology (Toronto, Ont.)·2026

Related Experiment Video

Updated: May 24, 2025

Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms
05:12

Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms

Published on: February 2, 2024

663

RNA-seq analysis reveals transcriptome changes in livers from Efcab4b knockout mice.

Chew W Cheng1, Lucia Pedicini1, Cintli Morales Alcala1

  • 1University of Leeds, Faculty of Medicine and Health, Leeds Institute of Cardiovascular and Metabolic Medicine, Leeds, LS2 9JT, UK.

Biochemistry and Biophysics Reports
|March 4, 2025
PubMed
Summary

Depleting EFCAB4B protein in mice resulted in larger livers and altered gene expression, impacting liver development and lipid metabolism. This suggests EFCAB4B influences susceptibility to liver injury and non-alcoholic fatty liver disease progression.

Keywords:
Hepatocarcinoma (HCC)Human EFCAB4B (Rab46 / CRACR2A)LiverMouse Efcab4bNon-alcoholic fatty liver disease (NAFLD)Non-alcoholic steatohepatitis (NASH)

More Related Videos

RNA-seq Analysis of Transcriptomes in Thrombin-treated and Control Human Pulmonary Microvascular Endothelial Cells
18:30

RNA-seq Analysis of Transcriptomes in Thrombin-treated and Control Human Pulmonary Microvascular Endothelial Cells

Published on: February 13, 2013

21.8K
Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis
12:44

Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis

Published on: November 11, 2014

12.3K

Related Experiment Videos

Last Updated: May 24, 2025

Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms
05:12

Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms

Published on: February 2, 2024

663
RNA-seq Analysis of Transcriptomes in Thrombin-treated and Control Human Pulmonary Microvascular Endothelial Cells
18:30

RNA-seq Analysis of Transcriptomes in Thrombin-treated and Control Human Pulmonary Microvascular Endothelial Cells

Published on: February 13, 2013

21.8K
Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis
12:44

Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis

Published on: November 11, 2014

12.3K

Area of Science:

  • Molecular biology
  • Genetics
  • Hepatology

Background:

  • EFCAB4B encodes Rab46 and CRACR2A, with variants linked to non-alcoholic fatty liver disease (NAFLD) progression.
  • Genome-wide association studies suggest a role for EFCAB4B in NAFLD.

Purpose of the Study:

  • To investigate the function of EFCAB4B in liver physiology and its potential role in NAFLD.
  • To identify molecular mechanisms underlying liver changes associated with EFCAB4B depletion.

Main Methods:

  • Global gene expression analysis using RNA-sequencing (RNA-seq) in EFCAB4B-depleted mice and wild-type (WT) controls.
  • Comparative analysis of liver tissues to identify differentially expressed genes (DEGs).
  • Bioinformatic analysis to determine enriched biological processes and pathways.

Main Results:

  • Mice globally depleted of EFCAB4B exhibited significantly larger livers compared to WT mice.
  • RNA-seq identified 69 differentially expressed genes, with enrichment in liver and bile development pathways.
  • DEGs were implicated in lipid metabolism, inflammation, ER stress, and fibrosis, pathways relevant to NAFLD progression to NASH and HCC.

Conclusions:

  • EFCAB4B plays a role in regulating liver size and gene expression.
  • EFCAB4B depletion impacts molecular pathways associated with liver injury and NAFLD progression.
  • These findings offer insights into the molecular mechanisms of EFCAB4B in liver disease susceptibility.