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Updated: May 24, 2025
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Isolation and Time-Lapse Imaging of Primary Mouse Embryonic Palatal Mesenchyme Cells to Analyze Collective Movement Attributes
Published on: February 13, 2021
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Single Cell Spatial Transcriptomics of the Murine Embryonic Palate Links Pax9 to Patterning and Organization of
Jeremie Oliver Piña1, Resmi Raju1, Evan Stipano1
1Section on Craniofacial Genetic Disorders, Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD), National Institutes of Health (NIH), Bethesda, MD, USA.
Research Square
|March 4, 2025
Summary
This study reveals disrupted Wnt signaling and extracellular matrix gene expression in cleft palate development using advanced spatial transcriptomics. These findings offer new molecular targets for potential therapies for human palatal clefts.
Area of Science:
- Developmental Biology
- Genomics
- Molecular Biology
Background:
- Palatal clefts result from complex morphological disruptions.
- The precise signaling microenvironment perturbations in palatal clefts are not fully understood.
Purpose of the Study:
- To investigate the genomic basis of cleft palate formation.
- To identify molecular targets for potential therapies for human palatal clefts.
Main Methods:
- Utilized the Pax9-/- murine model for cleft palate defect study.
- Employed Visium HD for single-cell resolution spatially resolved transcriptomics.
- Validated spatial gene expression using Xenium In Situ mRNA spatial profiling and RNAscope Multiplex assays.
Main Results:
- Identified palate cell-specific perturbation in Wnt signaling effector function.
- Observed disrupted expression of extracellular matrix genes in developing mesenchyme.
- Generated a comprehensive dataset for identifying key molecular targets.
Conclusions:
- The study provides a framework for translational research into cleft palate therapies.
- Disrupted Wnt signaling and extracellular matrix genes are key factors in cleft palate development.
- Advanced spatial transcriptomics offers novel insights into developmental defects.
