Visible Blue Light Does Not Induce DNA Damage in Human Dermal Fibroblasts

Photobiomodulation (PBM) is a noninvasive treatment modality that utilizes light to influence cellular activity. PBM has applications in various dermatological conditions. As blue light (BL, 400-500 nm) therapy gains popularity, concerns about its potential to induce DNA damage remain. This study investigates the effects of fluorescent BL (417 ± 5 nm) on human dermal fibroblast DNA, specifically examining the formation of cyclobutane pyrimidine dimers (CPD) and 6-4 photoproducts (6-4PPs) at fluences of 10, 30, and 45 J/cm². Our results demonstrate that BL exposure does not induce detectable DNA damage, in contrast to the well-documented effects of ultraviolet light, which is known to cause such damage. These findings support the safety of fluorescent BL therapy and align with prior research on LED-red and LED-near-infrared wavelengths. Continued clinical investigation of the effects of BL on skin will add to the understanding of the safety profile.