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Ovarian superstimulation protocols modulate the morphological phenotypes in bovine oviduct
Patricia Kubo Fontes1, Ana Paula Marques Andrade2, Laura Chuba Machado Rolniche2
1Center for Natural and Human Sciences, Federal University of ABC, Santo André, São Paulo, Brazil.
Theriogenology
|March 4, 2025
Summary
Ovarian superstimulation (OVS) protocols alter bovine oviduct morphology. Both protocols changed isthmus and ampulla structures, with the FSH/eCG protocol showing distinct effects on collagen and mucin levels.
Area of Science:
- Reproductive Biology
- Veterinary Science
- Animal Science
Background:
- Estradiol (E2) and progesterone (P4) significantly influence bovine oviduct morphology.
- Ovarian superstimulation (OVS) protocols are known to increase E2 levels in the bovine oviduct.
Purpose of the Study:
- To investigate the effects of two OVS protocols (FSH-only and FSH/eCG) on bovine oviduct morphology.
- To compare these effects against a control group (synchronized estrus).
Main Methods:
- Morphometric analysis of oviduct ampulla and isthmus segments.
- Collagen quantification (Picrosirius Red staining).
- Mucin quantification (Periodic Acid-Schiff/Alcian Blue staining).
- Fractal dimension analysis.
Main Results:
- Both OVS protocols decreased isthmus mucosal height, epithelial area, and luminal area, increasing collagen.
- FSH/eCG protocol increased ampulla muscle layer area and mucosal height, decreasing collagen.
- OVS treatments increased cell organization in both segments.
- FSH/eCG protocol increased mucin abundance in both segments.
Conclusions:
- OVS protocols induce a distinct morphological phenotype in the bovine oviduct.
- Changes include alterations in morphometrics, collagen, and mucin content.
- Specific OVS protocols elicit differential responses in oviduct segments.
