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Updated: May 24, 2025

Inducing Hairy Roots by Agrobacterium rhizogenes-Mediated Transformation in Tartary Buckwheat Fagopyrum tataricum
Published on: March 11, 2020
FtbHLH1, a transcription factor that interacts with FtATG8a, enhances the drought stress response in Tartary
Shuang Wang1, XinYi Cao2, Hongyou Li3
1College of Life Science, Sichuan Agricultural University, Ya'an, 625014, China; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agriculture University, Chengdu, 611130, China.
Abstract:
Tartary buckwheat (Fagopyrum tataricum) is a traditional cereal crop cultivated in hilly, arid, cool mountainous regions. The bHLH transcription factors play a pivotal role in regulating flavonoid metabolism and enhancing resistance to extreme environments in Tartary buckwheat. However, the functional characterization of bHLH genes in this species remains incomplete. Previous research identified FtbHLH1 as an interacting partner of the key autophagy protein FtATG8a through yeast library screening. Yeast two-hybrid, bimolecular fluorescence complementation, and luciferase complementation imaging assays confirmed that FtbHLH1 interacts with FtATG8a. This interaction depends on the AIM motifs (LEWYYL and QSWHFV) present in FtbHLH1, with both proteins co-localizing in the nucleus. The expression of FtbHLH1 was significantly induced by drought stress (P < 0.05), and its overexpression led to increased drought tolerance in transgenic Tartary buckwheat hairy roots. RNA sequencing revealed that FtbHLH1 up-regulated genes associated with stress response (e.g., FtCu/ZnSOD) as well as those involved in abscisic acid and methyl jasmonate biosynthesis and signaling pathways (e.g., FtCYP707As, FtRD29B, and FtJAZs). Further analysis indicated that the overexpression of FtbHLH1 enhances drought stress tolerance by altering the activities of antioxidant enzymes and promoting proline accumulation in both transgenic Arabidopsis and Tartary buckwheat hairy roots. This study provides theoretical support for selecting drought-resistant Tartary buckwheat varieties by elucidating the role of FtbHLH1 in the response to drought stress.
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Transcription
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Responses to Drought and Flooding
Responses to Heat and Cold Stress
Adaptations that Reduce Water Loss
General Transcription Factors
Regulation of Transpiration by Stomata

