Fragmentation pattern-based nontargeted screening strategy uncovered novel halogenated nucleotides in drinking water
Guangrong Sun1, Pei Guo2, Rong Liu2
1College of Environmental Science and Engineering, Yangzhou University, Yangzhou 225127, China; Department of Environmental Science, Zhejiang University, Hangzhou 310058, China.
Journal of Hazardous Materials
|March 4, 2025
Summary
A new method effectively identifies potentially harmful halogenated nucleotides in drinking water. This approach uses mass spectrometry to detect these disinfection byproducts, revealing chlorinated compounds form most readily and can be cytotoxic.
Area of Science:
- Environmental Chemistry
- Analytical Chemistry
- Toxicology
Background:
- Halogenated nucleotides are emerging disinfection byproducts in drinking water.
- Their identification is challenging due to high risk, diverse structures, and lack of standards.
Purpose of the Study:
- To develop an efficient method for identifying halogenated nucleotides in complex environmental samples.
- To assess the formation potential and cytotoxicity of these compounds.
Main Methods:
- A fragmentation pattern-based, nontargeted screening strategy using high-resolution mass spectrometry.
- Combined halogen isotope features and characteristic product ions (m/z 78.96, 96.97, 211.00) for identification.
- Cytotoxicity testing of 8-chloroadenosine 5'-monophosphate.
Main Results:
- Tentatively identified 72 halogenated nucleotides, including 9 novel brominated, iodinated, and mixed halogen compounds.
- Chlorinated nucleotides showed the highest formation potential, followed by brominated and iodinated.
- 8-Chloroadenosine 5'-monophosphate exhibited relatively high cytotoxicity.
Conclusions:
- The developed strategy enables effective nontargeted identification of unknown halogenated nucleotides.
- This method has broad applications for environmental sample analysis.
- Findings highlight the potential risks associated with halogenated nucleotides in drinking water.
Keywords:
Characteristic fragmentationCytotoxicityDisinfection byproductsHalogenated nucleotidesNontargeted identification
