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Updated: May 24, 2025
Development of Amelogenin-chitosan Hydrogel for In Vitro Enamel Regrowth with a Dense Interface
Published on: July 10, 2014
A novel biomimetic strategy for mimicking amelogenesis to repair enamel
Xu Chen1, Cheng Zhi1, Xinye Zhou1
1Tianjin Medical University School and Hospital of Stomatology & Tianjin Key Laboratory of Oral Soft and Hard Tissues Restoration and Regeneration, No.12 Qixiangtai Road, Heping District, Tianjin 300070, PR China; Tianjin Medical University Institute of Stomatology, No.12 Qixiangtai Road, Heping District, Tianjin 300070, PR China.
Abstract:
According to the principle of minimal invasiveness in modern dentistry, biomimetic remineralization therapy constitutes a significant strategy for the prevention and treatment of early enamel caries. Based on the three "key events" of amelogenesis in vivo, silk fibroin (SF) combined with carboxymethyl chitosan (CMC) successfully formed an SF/CMC composite, and amorphous calcium phosphate (ACP) was then used to form an SF/CMC-ACP nanocomposite with remineralization properties. In our study, SF was used as a template protein for biomimetic amelogenin, ACP was stabilized with CMC and the remineralization was guided using NaClO to simulate the action of proteolytic enzymes. The SF/CMC-ACP nanocomposite demonstrated excellent biocompatibility and enamel remineralization effects in both in vitro/in vivo experiments; thus, a theoretical basis for biomimetic enamel remineralization studies was provided.
