Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 24, 2025

Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus
10:49

Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus

Published on: January 28, 2019

8.5K

Comparative in silico and in vivo study of the antioxidant activity of lactoferrin, Geobacillus stearothermophilus,

Tarek A M Ismail1, Naglaa Elshafey2, Shehab E Talat1

  • 1Microbiology Department, Egyptian Drug Authority (EDA), Formerly National Organization of Drug Control and Research, Cairo, Egypt.

BMC Microbiology
|March 4, 2025
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Diaryl terephthalamides as small-molecule disruptors of the c-Myb-CBP/p300 KIX protein-protein interaction: Mechanistic insights and biochemical validation.

Biochemical and biophysical research communications·2026
Same author

Growth, yield and fruit quality of 'African Rose' plum as affected by three rootstocks.

BMC plant biology·2026
Same author

Nitrogen fertilization amplifies stem borer susceptibility in rice: genotype-dependent trade-offs between yield and resistance.

Frontiers in plant science·2026
Same author

Phenotypic profile of multi-drug-resistant Klebsiella spp. isolated from urinary tract and wound infections in Togo.

Scientific reports·2026
Same author

Phytochemical profile and bioactivities of hydroethanolic extracts of Pteleopsis suberosa and Piliostigma thonningii used in traditional medicine in Togo.

Scientific reports·2026
Same author

Genome-wide identification and expression analysis of Aspartic proteases in Hordeum vulagare L. reveals candidates involved in salt and drought tolerance.

Protoplasma·2026

Lactoferrin and specific bacterial strains show antioxidant and antiviral properties against Rotavirus. Lactoferrin significantly reduced Rotavirus

Area of Science:

  • Microbiology
  • Immunology
  • Biochemistry

Background:

  • Rotavirus is a leading cause of pediatric gastroenteritis with limited treatment options.
  • Investigating natural compounds for antiviral activity against Rotavirus is crucial.

Purpose of the Study:

  • To evaluate the antioxidant and antiviral potential of lactoferrin, Geobacillus stearothermophilus, and Lactobacillus delbrueckii subsp. lactis against Rotavirus.
  • To assess the therapeutic efficacy of these agents in a mouse model.

Main Methods:

  • Isolation and molecular identification of Geobacillus stearothermophilus and Lactobacillus delbrueckii subsp. lactis.
  • Antioxidant activity assay using DPPH.
  • In vivo study in mice involving oral administration of agents followed by Rotavirus infection.
Keywords:
Geobacillus stearothermophilusLactobacillus delbrueckiiRotavirusLactoferrinTNF-α IL-6

More Related Videos

Probiotic Studies in Neonatal Mice Using Gavage
10:36

Probiotic Studies in Neonatal Mice Using Gavage

Published on: January 27, 2019

18.5K
A Method to Assess Bacteriocin Effects on the Gut Microbiota of Mice
07:54

A Method to Assess Bacteriocin Effects on the Gut Microbiota of Mice

Published on: July 25, 2017

14.2K

Related Experiment Videos

Last Updated: May 24, 2025

Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus
10:49

Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus

Published on: January 28, 2019

8.5K
Probiotic Studies in Neonatal Mice Using Gavage
10:36

Probiotic Studies in Neonatal Mice Using Gavage

Published on: January 27, 2019

18.5K
A Method to Assess Bacteriocin Effects on the Gut Microbiota of Mice
07:54

A Method to Assess Bacteriocin Effects on the Gut Microbiota of Mice

Published on: July 25, 2017

14.2K
  • Measurement of inflammatory cytokines (TNF-α, IL-6) and histological examination of intestinal tissues.
  • Molecular docking studies of bacteriocins with Rotavirus VP6 protein.

    • Main Results:

    • Lactoferrin demonstrated the strongest antioxidant activity, followed by the bacterial isolates.
    • Lactoferrin treatment significantly reduced Rotavirus-induced inflammation and prevented intestinal damage in mice.
    • Geobacillus stearothermophilus and Lactobacillus delbrueckii subsp. lactis showed protective effects against Rotavirus pathology.
    • Molecular docking revealed high binding affinity of bacteriocins to Rotavirus VP6 protein, suggesting therapeutic potential.

    Conclusions:

    • Lactoferrin exhibits significant antiviral effects against Rotavirus, mitigating pathological changes and normalizing inflammatory responses.
    • Bacteriocins show promise as novel therapeutic agents for Rotavirus infections due to their strong binding to viral proteins.