Cellular-Resolution Image-Guided Localization in the Primate Brain.

Area of Science:

• Neuroscience
• Microscopy
• Computer Vision

Background:

• Accurate imaging of non-human primate brain tissue is crucial for understanding neural circuits.
• Flexible positioning of fluorescence microscopes is needed for comprehensive cortical mapping.

Purpose of the Study:

• To develop and validate a computer-vision pipeline for accurate, real-time localization of brain tissue during fluorescence microscopy.
• To enable flexible positioning of microscopes for imaging across the dorsal cortical convexity.

Main Methods:

• Utilized a fluorescence microscope on a parallel-kinematic stage.
• Developed a computer-vision pipeline for real-time localization.
• Referenced prior imaging sessions from different days for accuracy.

Main Results:

• Achieved accurate localization of 10-20 µm.
• Enabled real-time imaging within 10-20 seconds.
• Demonstrated successful application in non-human primate dorsal cortical convexity imaging.

Conclusions:

• The computer-vision pipeline facilitates flexible and accurate multi-session brain tissue imaging.
• This technology enhances non-human primate neuroscience research by improving spatial registration.
• Real-time localization is key for efficient and precise in-vivo or ex-vivo brain mapping.