Cellular-Resolution Image-Guided Localization in the Primate Brain

A fluorescence microscope mounted on a parallel-kinematic stage can be flexibly positioned to image brain tissue across the dorsal cortical convexity of the non-human primate. We introduce a computer-vision pipeline that enables accurate, 10-20 µm, real-time, 10-20 s, localization with reference to prior imaging sessions obtained on different days.