Bovine Embryo Development Monitoring Using Differential Electrical Impedance Spectroscopy

This study presents the development of a novel microfluidic device designed for non-invasive impedance spectroscopy assessment of mammalian embryos, with a focus on bovine embryos. The system is capable of gentle embryo manipulation using precisely controlled negative pressure. The microfluidic device includes adjustable electrodes for measuring electrical properties directly. It is also designed to facilitate embryo recovery after impedance measurements for immediate transfer into the mother. Simulations were performed to optimize the electrode size, with the aim of maximizing electrical current flow through the embryo. A decrease in the real component of the differential impedance at 1.49 MHz was observed and is correlated with the growth of the embryo's cell mass over time.