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Updated: May 24, 2025

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy
Published on: May 18, 2011
Resolution enhancement of total internal reflection microscopy via polarised scattering
Chenguang Liu1,2, Zhao Chen1, Zhenlong Xu1
1Advanced Microscopy and Instrumentation Research Center, School of Instrumentation Science and Engineering, Harbin Institute of Technology, Harbin, China.
Abstract:
This paper presents a novel approach for enhancing the resolution of label-free total internal reflection microscopy. This method leverages the directional difference of the sample polar scattering by capturing microscopic images under the illumination of evanescent waves propagating in various directions. By computing the high-order autocorrelation accumulation of these images at different angles, we achieved a 1.5-fold improvement in resolution while effectively reducing the distortion caused by laser speckles and image artefacts. This technique holds promise for long-term, noninvasive observation with enhanced resolution.
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