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Updated: Jun 13, 2025

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Chitosan double-stranded RNA nanocomplexes for Piezodorus guildinii control.

Claudia Schvartzman, Sara Murchio1, Analía Castro2

  • 1Área de Mejoramiento Genético Vegetal y Biotecnología, Instituto Nacional de Investigación Agropecuaria (INIA), Wilson Ferreira Aldunate Research Station, Canelones, Uruguay.

Pest Management Science
|March 5, 2025
PubMed
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RNA interference (RNAi) using double-stranded RNA (dsRNA) effectively reduced Piezodorus guildinii viability. Chitosan-dsRNA nanocomplexes improved stability and efficacy for integrated pest management of this soybean pest.

Area of Science:

  • Agricultural Entomology
  • Molecular Biology
  • Nanotechnology

Background:

  • Piezodorus guildinii infestation significantly reduces soybean yield, grain quality, and germination.
  • RNA interference (RNAi) offers a safe, targeted approach for pest management.
  • dsRNA conjugation with polymers like chitosan enhances stability and efficacy.

Purpose of the Study:

  • To assess the impact of dsRNA administration on P. guildinii viability.
  • To evaluate dsRNA-chitosan nanocomplexes as a pest control strategy.

Main Methods:

  • Synthesis and administration of dsRNAs targeting specific genes in P. guildinii.
  • Evaluation of insect mortality via injection and ingestion assays.
  • Development and in vitro/in vivo testing of dsRNA-chitosan nanocomplexes.
Keywords:
RNAigene silencinghemipterapest controlstink bugs

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  • Gene expression analysis using RT-qPCR.

    • Main Results:

    • dsRNA administration resulted in significant mortality rates (76% via injection, 49% via ingestion).
    • Chitosan-dsRNA nanocomplexes showed enhanced stability and a 66% cumulative corrected mortality rate in vivo.
    • Targeted gene expression was significantly reduced post-treatment.

    Conclusions:

    • dsRNA administration effectively reduces P. guildinii viability and silences target genes.
    • Chitosan-dsRNA nanoparticles offer improved stability and efficacy for pest control.
    • These findings support the development of novel integrated management strategies for stink bug control.