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Related Concept Videos

Cell Migration01:09

Cell Migration

16.9K
Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.
16.9K
Role of Myosin in Cell Migration01:18

Role of Myosin in Cell Migration

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Myosins are multimeric motor proteins involved in various cellular processes such as migration, adhesion, and proliferation. Myosin II is the most common type in animal cells, which binds and cross-links actin filaments.
Myosin II  is a hexamer comprising two heavy chains with globular heads and coiled-coil tails, two regulatory light chains, and two essential light chains. The ATPase sites on the myosin heads hydrolyze ATP, and the released phosphate generates the force for contraction....
2.2K
Cancer Cell Migration through Invadopodia01:35

Cancer Cell Migration through Invadopodia

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Invadosome is a broad category of cell surface structures with proteolytic activity that  degrades the extracellular matrix (ECM). Invadosomes are present in normal cell types, including macrophages, endothelial cells, and neurons, as well as tumor cells. Although the macrophage podosomes and tumor cell invadopodia are classified as invadosomes, they have different structures, molecular pathways, and functions. Podosomes are short structures that last for a few minutes. However,...
2.3K
Mechanism of Lamellipodia Formation01:31

Mechanism of Lamellipodia Formation

2.5K
Cells migrating in response to external stimuli form lamellipodia, which are thin membrane protrusions supported by a mesh of linked, branched, or unbranched actin filaments. These actin filaments interact with myosin motor proteins, creating the dynamic actomyosin complex within the cytoskeleton. Contractility, or the ability to generate contractile stress, is inherent to the actomyosin complex. It helps cells detect the stiffness of the surrounding ECM and exert contractile force for...
2.5K
Cytoskeletal Coordination in Cell Migration01:32

Cytoskeletal Coordination in Cell Migration

4.7K
A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker...
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Cell Polarization by Rho Proteins01:21

Cell Polarization by Rho Proteins

2.6K
Cell polarity is the asymmetric distribution of cellular and membrane components, making one side of the cell different from the other. This polarity is essential to many processes such as embryogenesis, axon migration, glucose transport across epithelial cells, and directional cell migration. A migrating cell responds to intracellular or extracellular signals via molecular cascades that reorganize the actin cytoskeleton to establish this polarity. In these cells, the Rho family proteins Cdc42,...
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Related Experiment Video

Updated: May 24, 2025

Analysis of Cell Migration within a Three-dimensional Collagen Matrix
08:02

Analysis of Cell Migration within a Three-dimensional Collagen Matrix

Published on: October 5, 2014

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Type III Collagen Promotes Pseudopodium-Driven Cell Migration.

Ruiwen Fu1,2, Kuangzheng Zhu3, Zhouyang Li1

  • 1ZJU-Hangzhou Global Scientific and Technological Innovation Center, Zhejiang University, Hangzhou, Zhejiang 311200, China.

Chem & Bio Engineering
|March 5, 2025
PubMed
Summary
This summary is machine-generated.

Recombinant human type III collagen (hCOL3) enhances cell migration by increasing pseudopodia and filopodia. This collagen fragment boosts cell speed, diffusion, and directionality, potentially improving wound healing.

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Related Experiment Videos

Last Updated: May 24, 2025

Analysis of Cell Migration within a Three-dimensional Collagen Matrix
08:02

Analysis of Cell Migration within a Three-dimensional Collagen Matrix

Published on: October 5, 2014

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Concentric Gel System to Study the Biophysical Role of Matrix Microenvironment on 3D Cell Migration
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Concentric Gel System to Study the Biophysical Role of Matrix Microenvironment on 3D Cell Migration

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Measuring Cell-Edge Protrusion Dynamics during Spreading using Live-Cell Microscopy
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Area of Science:

  • Biochemistry
  • Cell Biology
  • Biomaterials Science

Background:

  • The extracellular matrix (ECM), especially collagen, influences cell migration, but mechanisms are unclear.
  • Pseudopodium formation and cell motility dynamics require further investigation.

Purpose of the Study:

  • To investigate the impact of recombinant human type III collagen (hCOL3) on cell migration.
  • To analyze hCOL3's effect on pseudopodium dynamics and cell motility.

Main Methods:

  • Utilized single-cell and collective-cell migration assays.
  • Employed scanning electron microscopy to observe cell morphology.
  • Studied a fragmented form of hCOL3 engineered for research.

Main Results:

  • hCOL3 significantly increased cell migration velocity and effective diffusion coefficient.
  • Enhanced directionality was observed in both single-cell and collective migration.
  • Scanning electron microscopy showed an increased number and length of filopodia upon hCOL3 treatment.

Conclusions:

  • hCOL3 promotes more targeted and rapid cell migration.
  • Increased filopodia enhance environmental cue detection and cell extension.
  • Findings suggest potential applications in accelerating wound healing processes.