Nucleic Acid Framework-Enabled Spatial Organization for Biological Applications

Nucleic acid frameworks (NAFs) are artificially prepared from natural nucleic acids with a precise size and structure. DNA origami exhibits controllable 2D lamellar structure and thus is easily used to construct 3D structures with different morphologies. Tetrahedral DNA nanostructures (TDNs) are prepared with four DNA strands that hybridize to each other with a tetrahedral structure. Here we summarize molecular spatial organization with DNA origami and TDNs as models for 2D- and 3D-recombinations, discuss NAF-based biomimicking of proteins and biomembranes, and introduce the identification probes, functional groups, and intercalators for biosensing, bioimaging, and nanomedicine therapy. NAFs are also extended to applications to guide the formation of inorganic nanoparticles with precise size and structure. Thus, the NAFs exhibit special organization, are easy to functionalize, and are becoming an important platform for interdisciplinary study and applications, such as nanotechnology, biochemistry, synthetic biology, and nanomedicine.