Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Feedback Regulation of Calcium Concentration01:27

Feedback Regulation of Calcium Concentration

3.3K
Calcium is an essential signaling molecule required for various cellular functions. Calcium pumps and ion channels on cell and organellar membranes, such as those on the endoplasmic reticulum (ER), regulate calcium concentrations inside the cell. They remain closed, keeping the cytosolic calcium levels low at a resting state.
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
3.3K
Cell-matrix's Response to Mechanical Forces01:13

Cell-matrix's Response to Mechanical Forces

2.5K
In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue. 
Anchoring junctions mechanically attach a cell to the...
2.5K
Smooth Muscle Contraction01:25

Smooth Muscle Contraction

2.3K
Smooth muscle contraction is a complex process vital for various bodily functions, from maintaining blood vessel tension to facilitating the movement of food through the digestive tract. Unlike striated muscles, smooth muscle contraction begins more slowly and lasts longer.
The onset of contraction is triggered by an increase in calcium ions within the sarcoplasm, similar to the process in striated muscle. However, smooth muscles have a relatively smaller reservoir of the sarcoplasmic...
2.3K
Calmodulin-dependent Signaling01:16

Calmodulin-dependent Signaling

5.1K
Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
5.1K
Tension Response at Adherens Junctions01:26

Tension Response at Adherens Junctions

2.6K
The adherens junctions that anchor cells together are multi-protein complexes that dynamically adapt to mechanical stimuli such as tensile forces and shear stress. Mechanosensory proteins in these junctions can sense such mechanical stimuli and undergo a shift in their conformation, resulting in an altered function — a process called mechanotransduction.
α-Catenin as a Mechanosensory Protein
The α-catenin of adherens junctions is an allosteric protein with three VH (vinculin...
2.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Successful Treatment of Lymphocele after Inguinal Lymph Node Dissection with OK-432 Sclerotherapy: A Report of Two Cases.

Journal of plastic and reconstructive surgery·2026
Same author

Surgical methods for reducing early wound dehiscence after reconstruction surgery for pressure ulcer.

Journal of wound care·2026
Same author

Current Status and Potential of Online Telemedicine in Plastic Surgery.

Journal of plastic and reconstructive surgery·2025
Same author

A New Class of Vitamin K Analogues Containing the Side Chain of Retinoic Acid Have Enhanced Activity for Inducing Neuronal Differentiation.

ACS chemical neuroscience·2025
Same author

Suitability of the Microvascular Anastomotic Coupler System for Small Spliced Vein Grafts.

Journal of plastic and reconstructive surgery·2025
Same author

Plantar pressure and shear stress during gait in people with diabetic neuropathy.

Diabetology international·2025

Related Experiment Video

Updated: May 24, 2025

Author Spotlight: Advancements in Cell and Tissue Engineering for Tendon Repair
04:48

Author Spotlight: Advancements in Cell and Tissue Engineering for Tendon Repair

Published on: March 1, 2024

1.6K

Specific Calcium Signal Responses in Human Keloid-Derived Fibroblasts During Cyclical Stretching: Basic Research.

Kazuhide Mineda1, Katsuya Sato2, Tasuku Nakahara3

  • 1Plastic and Reconstructive Surgery, School of Medicine Tokushima University Tokushima City Japan.

Health Science Reports
|March 5, 2025
PubMed
Summary

Keloid fibroblasts show higher calcium spike amplitudes than normal cells during prolonged stretching. This suggests a specific subpopulation of keloid cells may drive keloid formation through abnormal calcium signaling.

Keywords:
calcium oscillationcalcium signal responsecalcium spikecyclical stretchingintracellular Ca2+keloid

More Related Videos

Live Cell Imaging during Mechanical Stretch
07:42

Live Cell Imaging during Mechanical Stretch

Published on: August 19, 2015

10.3K
A Novel Stretching Platform for Applications in Cell and Tissue Mechanobiology
16:46

A Novel Stretching Platform for Applications in Cell and Tissue Mechanobiology

Published on: June 3, 2014

11.7K

Related Experiment Videos

Last Updated: May 24, 2025

Author Spotlight: Advancements in Cell and Tissue Engineering for Tendon Repair
04:48

Author Spotlight: Advancements in Cell and Tissue Engineering for Tendon Repair

Published on: March 1, 2024

1.6K
Live Cell Imaging during Mechanical Stretch
07:42

Live Cell Imaging during Mechanical Stretch

Published on: August 19, 2015

10.3K
A Novel Stretching Platform for Applications in Cell and Tissue Mechanobiology
16:46

A Novel Stretching Platform for Applications in Cell and Tissue Mechanobiology

Published on: June 3, 2014

11.7K

Area of Science:

  • Dermatology
  • Cell Biology
  • Biophysics

Background:

  • Keloids often form in skin areas subjected to constant stretching.
  • While some keloid fibroblasts show increased calcium spikes with short-term stretching, their response to long-term stretching is not well understood.

Purpose of the Study:

  • To compare intracellular calcium (Ca2+) dynamics in keloid-derived fibroblasts versus normal dermal fibroblasts under prolonged cyclical stretching.
  • To investigate the role of calcium signaling in keloid pathogenesis.

Main Methods:

  • Normal and keloid fibroblasts were subjected to 10% strain cyclical stretching for 30 minutes.
  • Intracellular Ca2+ levels were measured using confocal laser microscopy and fluorescence intensity ratios.
  • Calcium spikes were defined as a transient ratio increase of ≥20%.

Main Results:

  • No significant difference in the rate of calcium signal response-positive cells was found between keloid and control groups (26.9% vs. 25.0%).
  • Keloid-derived fibroblasts exhibited significantly higher calcium spike amplitudes compared to normal fibroblasts (1.66 vs. 1.41).
  • A subset of keloid fibroblasts (9.6%) displayed multiple hypercalcium spikes, with a cutoff value of 2.12.

Conclusions:

  • Prolonged stretching leads to significantly higher calcium spike amplitudes in keloid fibroblasts.
  • A distinct subpopulation of keloid fibroblasts with hyperactive calcium signaling may contribute to keloid formation.
  • Further research is warranted to explore the link between this subpopulation and keloid pathogenesis, including collagen overproduction, angiogenesis, and inflammation.