Related Experiment Video

Updated: May 24, 2025

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
09:44

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

Published on: December 23, 2022

2.1K

Draft genome sequence of emerging pathogen Morganella morganii strain PS00513 isolated from a chronic surgical wound

Kira N Allison1, Rayhane Mejlaoui1, Katrina G DeZeeuw2

  • 1Department of Health Sciences, Carleton University, Ottawa, Ontario, Canada.

Microbiology Resource Announcements
|March 5, 2025
PubMed

Insights

We sequenced the genome of Morganella morganii strain PS00513, isolated from a surgical wound infection. The study identified multiple antibiotic resistance genes on both its chromosome and plasmid.

Area of Science:

  • Microbiology and Genomics
  • Infectious Diseases

Background:

  • Morganella morganii is an opportunistic pathogen frequently associated with healthcare-associated infections.
  • Understanding the genomic basis of antimicrobial resistance in M. morganii is crucial for effective treatment strategies.

Purpose of the Study:

  • To perform whole-genome sequencing of Morganella morganii strain PS00513.
  • To identify the antimicrobial resistance gene profile of this clinical isolate.
  • To characterize the genetic elements (chromosome and plasmid) carrying resistance genes.

Main Methods:

  • Draft genome sequencing of Morganella morganii strain PS00513.
  • Bioinformatic analysis to identify antimicrobial resistance genes.
  • Characterization of chromosomal and plasmid DNA sequences.

Main Results:

  • The draft genome sequence of M. morganii strain PS00513 comprises a 3.87 Mb chromosome and a 9.6 kb plasmid.
  • Multiple antimicrobial resistance genes were identified.
  • Both the chromosome and the plasmid harbor antibiotic resistance genes, indicating a complex resistance mechanism.

Conclusions:

  • The genomic data provides valuable insights into the genetic makeup of Morganella morganii.
  • The presence of multiple resistance genes on both chromosomal and plasmid DNA highlights the potential for rapid dissemination of antibiotic resistance.
  • This study contributes to understanding the antimicrobial resistance landscape of M. morganii in clinical settings.
Keywords:
Oxford Nanopore sequencingantimicrobial resistancebiofilmschronic woundsurgical wound

More Related Videos

Author Spotlight: A Cost-Effective Genomic Workflow for Advancing Rabies Control in Resource-Limited Settings10:26

Author Spotlight: A Cost-Effective Genomic Workflow for Advancing Rabies Control in Resource-Limited Settings

Published on: August 18, 2023

5.0K
Forward Genetic Approaches in Chlamydia trachomatis09:03

Forward Genetic Approaches in Chlamydia trachomatis

Published on: October 23, 2013

12.8K

Related Concept Videos

Genomics02:02

Genomics

Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
35.6K
Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
86.9K
Genomic DNA in Prokaryotes00:46

Genomic DNA in Prokaryotes

The genome of most prokaryotic organisms consists of double-stranded DNA organized into one circular chromosome in a region of cytoplasm called the nucleoid. The chromosome is tightly wound, or supercoiled, for efficient storage. Prokaryotes also contain other circular pieces of DNA called plasmids. These plasmids are smaller than the chromosome and often carry genes that confer adaptive functions, such as antibiotic resistance.
Genomic Diversity in Bacteria
Although bacterial genomes are much...
43.3K
Genome Size and the Evolution of New Genes03:21

Genome Size and the Evolution of New Genes

While every living organism has a genome of some kind (be it RNA, or DNA), there is considerable variation in the sizes of these blueprints. One major factor that impacts genome size is whether the organism is prokaryotic or eukaryotic. In prokaryotes, the genome contains little to no non-coding sequence, such that genes are tightly clustered in groups or operons sequentially along the chromosome. Conversely, the genes in eukaryotes are punctuated by long stretches of non-coding sequence.
7.8K
Synthetic Biology02:55

Synthetic Biology

Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
Golden rice
Golden rice is a genetically modified...
4.7K