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Complexometric Titration: Ligands

Complexometric Titration: Ligands

Different monodentate and polydentate ligands are used as complexing agents in complexometric titration reactions. The formation of complexes by mono- and bidentate ligands involves two or more intermediate steps, limiting their use as complexing agents. In comparison, polydentate ligands can form complexes with metal ions in a single-step process, facilitating sharper end points. This means polydentate ligands, such as amino carboxylic acid derivatives, are most commonly employed in...

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Updated: May 24, 2025

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Published on: September 19, 2017

Water-soluble luminescent platinum(II) complexes for guanine quadruplex binding.

Simon Kroos¹, Marian Hebenbrock¹, Alexander Hepp¹

¹Universität Münster, Institut für Anorganische und Analytische Chemie, Corrensstr. 28/30, 48149 Münster, Germany. mueller.j@uni-muenster.de.

Dalton Transactions (Cambridge, England : 2003)

|March 5, 2025

Summary

Researchers developed novel platinum(II) complexes for targeting guanine quadruplex (G4) DNA. Substituent modifications influence their binding affinity to G4 structures, offering potential for new therapeutic agents.

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Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

Area of Science:

Inorganic Chemistry
Medicinal Chemistry
Biophysical Chemistry

Background:

Guanine quadruplexes (G4) are non-canonical DNA secondary structures implicated in various biological processes.
Developing small molecules that selectively bind to G4 DNA is a key strategy for therapeutic intervention.
Platinum(II) complexes are known for their anticancer properties and ability to interact with DNA.

Purpose of the Study:

To synthesize and characterize a series of novel water-soluble platinum(II) complexes.
To investigate the binding interactions of these complexes with different guanine quadruplex (G4) DNA topologies.
To explore the structure-activity relationship between ligand substituents and G4 binding affinity.

Main Methods:

Synthesis and characterization of 16 platinum(II) complexes with a common tridentate N^N^C-donor ligand and varied ancillary ligands.
Single-crystal X-ray diffraction to confirm structural integrity and planarity of the complexes.
Spectroscopic techniques (luminescence, circular dichroism) and cryo-ESI mass spectrometry to study DNA-complex interactions.

Main Results:

The synthesized platinum(II) complexes exhibit water solubility and maintain structural features suitable for DNA intercalation.
Complexes demonstrated varying degrees of interaction with different G4 DNA structures.
A complex correlation was observed between the nature of substituents on the ligand and the binding efficacy with G4 DNA.

Conclusions:

The study successfully generated a library of platinum(II) complexes with tunable properties for G4 DNA recognition.
The findings highlight the importance of substituent design in optimizing G4-binding agents.
These luminescent platinum(II) complexes show promise as potential G4-targeting therapeutic agents.