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Advances in magnetic affinity-based isolation/detection of exosomes for robust diagnostics
Veronika Solovicová1, Anna Ďatková1, Tomáš Bertók1
1Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 5807/9, 845 38, Bratislava, Slovak Republic.
Mikrochimica Acta
|March 5, 2025
Summary
This review explores exosomes as disease biomarkers, focusing on magnetic affinity capture methods for isolating specific exosome subpopulations and their contents for diagnostic applications.
Area of Science:
- Biotechnology
- Biochemistry
- Nanomedicine
Background:
- Exosomes are key extracellular vesicles implicated in intercellular communication.
- Their potential as biomarkers for various diseases is increasingly recognized.
- Current isolation methods face challenges in specificity and efficiency.
Purpose of the Study:
- To provide a comprehensive overview of exosome isolation techniques.
- To focus on magnetic (bio)affinity capture for exosome detection and subpopulation isolation.
- To analyze analytical and clinical parameters of these methods.
Main Methods:
- Review of diverse exosome isolation strategies.
- Detailed examination of magnetic (bio)affinity capture techniques.
- Analysis of various affinity ligands including antibodies, aptamers, and peptides.
- Inclusion of glycan, transferrin, and phospholipid recognition methods.
- Consideration of electrostatic interactions for capture.
Main Results:
- Summary of magnetic (bio)affinity capture approaches for exosome and exosomal cargo detection.
- Extensive table detailing analytical and clinical parameters from 2023-2024 publications.
- Identification of specific sub-population isolation capabilities.
Conclusions:
- Magnetic (bio)affinity capture offers promising avenues for exosome isolation and biomarker determination.
- Discussion of the advantages and disadvantages of these methods.
- Highlighting the need for continued research in optimizing exosome-based diagnostics.
