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ATPS-enabled single-step printing of chemically and mechanically on-demand tunable perfusable channels in ejectable
Malin Becker1, Francisca Gomes1, Isa Porsul1
1Leijten Laboratory, Department of Developmental BioEngineering, TechMed Centre, University of Twente, Enschede 7522 NB, The Netherlands.
Biofabrication
|March 5, 2025
Summary
This study introduces a novel 3D bioprinting method using aqueous two-phase systems (ATPS) and supramolecular complexation. This technique enables the creation of tunable, perfusable engineered tissues from low-viscosity materials in a single step.
Area of Science:
- Biotechnology
- Materials Science
- Tissue Engineering
Background:
- Current 3D bioprinting relies on viscous materials, limiting design flexibility.
- Downstream modifications often require multi-step processes, increasing complexity.
Purpose of the Study:
- To develop a single-step 3D bioprinting method for low-viscosity materials.
- To enable simultaneous mechanical and chemical tuning of engineered constructs.
- To create intricate, perfusable, and potentially injectable biofabricated tissues.
Main Methods:
- Utilized aqueous two-phase system (ATPS) stabilized 3D bioprinting.
- Incorporated supramolecular complexation for material tunability.
- Introduced Dex-TAB as a versatile backbone material.
- Demonstrated fabrication of functionalized, interconnected tube-shaped constructs.
- Showcased direct printing into crosslinkable polymer solutions for pre-patterned constructs.
Main Results:
- Successfully fabricated intricate, perfusable engineered constructs using low-viscosity materials.
- Achieved single-step mechanical and chemical tunability of constructs.
- Demonstrated the creation of functionalized channels and pre-patterned perfusable structures.
- Generated constructs that are both mechanically robust and chemically tunable.
Conclusions:
- ATPS-enabled low-viscosity 3D bioprinting offers a versatile platform for biofabrication.
- This method facilitates the creation of highly functional, perfusable, and potentially injectable constructs.
- The approach advances applications in tissue engineering and regenerative medicine.
