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MiRNA expression profiling and clinical implications in prostate cancer across various stages

  • 0Department of Urology, The Second Affiliated Hospital of Nanchang University, No.1 Minde Road, Nanchang, 330006, Jiangxi, People's Republic of China.
Scientific Reports +

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March 5, 2025

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March 5, 2025

View abstract on PubMed

Summary

This summary is machine-generated.

This study identifies microRNA (miRNA) expression profiles in prostate cancer (PCa), finding hsa-miR-6715b-3p highly expressed in PCa tissues. This miRNA shows potential as a biomarker for PCa progression and therapy resistance.

Area Of Science

  • Oncology
  • Molecular Biology
  • Genetics

Background

  • Prostate cancer (PCa) is a significant global health concern.
  • MicroRNA (miRNA) dysregulation is implicated in various cancers, including PCa.
  • Identifying specific miRNA biomarkers is crucial for early detection and treatment monitoring.

Purpose Of The Study

  • To screen and identify miRNA expression profiles in different stages of PCa and benign prostatic hyperplasia (BPH).
  • To determine consistently differentially expressed miRNAs across PCa stages for potential biomarker discovery.
  • To identify novel miRNAs and validate the expression of a specific miRNA (hsa-miR-6715b-3p) in PCa.

Main Methods

  • High-throughput sequencing was employed to profile miRNA expression in 12 PCa and BPH specimens.
  • Bioinformatics analysis was used to identify differentially expressed miRNAs and predict novel miRNAs.
  • Gene Ontology (GO) and KEGG pathway enrichment analyses were performed on miRNA-derived genes.
  • Quantitative real-time PCR (qRT-PCR) was utilized to validate the expression of hsa-miR-6715b-3p.

Main Results

  • A total of 1526 miRNAs were identified, with 228 differentially expressed (100 upregulated, 128 downregulated).
  • 69 novel miRNAs were predicted.
  • hsa-miR-6715b-3p was found to be highly expressed in PCa tissues compared to BPH tissues.

Conclusions

  • This study identified distinct miRNA expression profiles in PCa and BPH.
  • hsa-miR-6715b-3p is a promising candidate biomarker for PCa progression, showing high expression in tumor tissues.
  • The findings provide a foundation for further research into the clinical utility of hsa-miR-6715b-3p in PCa diagnosis, prognosis, and therapy.

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