Related Experiment Video

Updated: May 24, 2025

Author Spotlight: Efficiently Eliminating Bacteriophages from Infected Salmonella Cultures Using Lipopolysaccharides
07:19

Author Spotlight: Efficiently Eliminating Bacteriophages from Infected Salmonella Cultures Using Lipopolysaccharides

Published on: June 28, 2024

770

Isolation and Characterization of Three Lytic Bacteriophages to Overcome Multidrug-, Extensive Drug-, and

Marwan Mahmood Saleh1, Majeed Arsheed Sabbah2, Zahraa Kamel Zedan3

  • 1Department of Biophysics, College of Applied Sciences, University of Anbar, Baghdad, Iraq.

PHAGE (New Rochelle, N.Y.)
|March 6, 2025
PubMed
Abstract

Insights

A novel phage cocktail effectively lyses multidrug-resistant (MDR) and extensively drug-resistant (XDR) Pseudomonas aeruginosa. This bacteriophage therapy shows promise as a safe, nonantibiotic alternative for treating resistant bacterial infections.

Area of Science:

  • Microbiology
  • Bacteriophage Therapy
  • Antimicrobial Resistance

Background:

  • The rise of multidrug-resistant (MDR) pathogens demands novel nonantibiotic therapeutics.
  • Bacteriophage (phage) therapy presents a promising alternative antimicrobial strategy.

Purpose of the Study:

  • To evaluate the in vitro lytic efficacy of a three-phage cocktail against multidrug-resistant (MDR), extensive drug-resistant (XDR), and pandrug-resistant (PDR) Pseudomonas aeruginosa isolates.

Main Methods:

  • A three-phage cocktail (MMS1, MMS2, MMS3) was generated using host range and genetic data.
  • The cocktail was purified via phage enrichment and tested against 114 P. aeruginosa isolates with variable antibiotic resistance.

Main Results:

  • The MMS phage cocktail demonstrated lytic activity against P. aeruginosa in both planktonic and dish cultures.
  • Phages remained viable across a temperature range of 4-50°C and pH 4-9.
  • Sequencing of MMS3 confirmed the absence of bacterial pathogenicity or antibiotic resistance genes.

Conclusions:

  • The phage cocktail effectively lysed XDR and PDR P. aeruginosa isolates, indicating its potential as an antibiotic substitute.
  • This bacteriophage approach does not promote antibiotic resistance and may be valuable for personalized phage therapeutics.
Keywords:
antibioticsinfectious diseaseslytic phagemultidrug resistancephagephage therapy

More Related Videos

Use of the Soft-agar Overlay Technique to Screen for Bacterially Produced Inhibitory Compounds06:38

Use of the Soft-agar Overlay Technique to Screen for Bacterially Produced Inhibitory Compounds

Published on: January 14, 2017

32.2K
Application of the Intelligent High-Throughput Antimicrobial Sensitivity Testing/Phage Screening System and Lar Index of Antimicrobial Resistance09:59

Application of the Intelligent High-Throughput Antimicrobial Sensitivity Testing/Phage Screening System and Lar Index of Antimicrobial Resistance

Published on: July 21, 2023

1.1K

Related Concept Videos

Lytic Cycle of Bacteriophages01:30

Lytic Cycle of Bacteriophages

Bacteriophages, also known as phages, are specialized viruses that infect bacteria. A key characteristic of phages is their distinctive “head-tail” morphology. A phage begins the infection process (i.e., lytic cycle) by attaching to the outside of a bacterial cell. Attachment is accomplished via proteins in the phage tail that bind to specific receptor proteins on the outer surface of the bacterium. The tail injects the phage’s DNA genome into the bacterial cytoplasm. In the...
70.1K
Antibiotic Selection00:57

Antibiotic Selection

Overview
52.1K
Defense Against Bacterial Pathogens01:31

Defense Against Bacterial Pathogens

The human immune system is a complex network of cells, tissues, and organs that work together to defend the body against bacterial infections. It consists of various immune cells, each playing a specific role in the defense mechanism.
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
1.4K
Lysogenic Cycle of Bacteriophages00:43

Lysogenic Cycle of Bacteriophages

In contrast to the lytic cycle, phages infecting bacteria via the lysogenic cycle do not immediately kill their host cell. Instead, they combine their genome with the host genome, allowing the bacteria to replicate the phage DNA along with the bacterial genome. The incorporated copy of the phage genome is called the prophage. Some prophages can re-activate and enter the lytic cycle. This often occurs in response to a perturbation, such as DNA damage, but can also transpire in the absence of...
61.7K