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Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform
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Microfluidic system for efficient molecular delivery to artificial cell membranes.

Arash Yahyazadeh Shourabi1, Martina Iacona1, Marie-Eve Aubin-Tam1

  • 1Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology, Van der Maasweg 9, Delft, 2629 HZ, The Netherlands. M.E.Aubin-Tam@tudelft.nl.

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Summary

This study presents a novel microfluidic device for stable freestanding lipid bilayers, enabling efficient molecular delivery and analysis of drug interactions with cell membranes. This platform advances drug screening and membrane biophysics research.

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Area of Science:

  • Biophysics
  • Biomedical Engineering
  • Materials Science

Background:

  • Cell membranes are critical drug targets, but in vitro drug screening platforms face challenges with membrane stability and buffer exchange.
  • Existing methods often use whole cells or tissues, limiting direct study of membrane-drug interactions.
  • Microfluidic systems offer potential but struggle with stable freestanding lipid bilayer formation and media manipulation.

Purpose of the Study:

  • To develop a novel microfluidic device for creating stable freestanding lipid bilayers.
  • To enable efficient molecular delivery and study drug interactions with these artificial membranes.
  • To characterize the impact of drugs on membrane biophysical properties.

Main Methods:

  • A new microfluidic chip design incorporating bubble traps and resistance channels for hydrodynamic control.
  • Formation of stable freestanding 1-palmitoyl-2-oleoyl-glycero-3-phosphocholine (POPC) lipid bilayers.
  • Delivery of the antibiotic azithromycin to the bilayers and characterization using optical tweezers.

Main Results:

  • Achieved stable freestanding lipid bilayers with controlled buffer replacement.
  • Successfully delivered azithromycin and observed its effects on membrane properties.
  • Quantified changes in membrane tension and bending rigidity induced by azithromycin.

Conclusions:

  • The developed microfluidic device provides a stable and versatile platform for studying membrane-drug interactions.
  • This technology facilitates molecular delivery and analysis of impacts on freestanding lipid bilayers.
  • It offers a valuable tool for advancing drug discovery and understanding membrane biophysics.