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Related Concept Videos

Bioreactor Controls-II01:18

Bioreactor Controls-II

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In aerobic fermentations, oxygen is vital for microbial growth and metabolite production. Since air comprises only about 20% oxygen and the gas is poorly soluble in water—just 9 ppm at 20°C—supplying sufficient oxygen becomes a critical challenge, especially in high-demand processes like yeast growth or citric acid production. Even a fully saturated broth may offer only a few seconds of oxygen availability.To address this, sterile or scrubbed air is introduced into the...
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A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments
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A Versatile and Modular Microfluidic System for Dynamic Cell Culture and Cellular Interactions.

Qasem Ramadan1, Rana Hazaymeh2, Mohammed Zourob1

  • 1College of Science & General Studies, Alfaisal University, Riyadh 11533, Saudi Arabia.

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|March 6, 2025
PubMed
Summary
This summary is machine-generated.

A novel modular microfluidic system enables dynamic cell co-culture, allowing flexible arrangements for studying cell-cell interactions and testing therapeutics in a physiologically relevant microenvironment.

Keywords:
fabricationintegrationmicrofluidicsorgan-on-a-chip

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Area of Science:

  • Biotechnology and Biomedical Engineering
  • Cell Biology
  • Microfluidics

Background:

  • Developing advanced cell culture models is crucial for understanding complex biological systems.
  • Microfluidic devices offer precise control over cellular microenvironments.
  • Existing co-culture systems often lack modularity and dynamic reconfiguration capabilities.

Purpose of the Study:

  • To develop a versatile and modular microfluidic system for dynamic cell co-culture.
  • To enable flexible interconnection and reconfiguration of microfluidic chips for studying cell-cell communication.
  • To assess the system's utility in modeling complex cellular interactions and evaluating therapeutic agents.

Main Methods:

  • Fabrication and assembly of modular microfluidic chips with dual compartments and porous membranes.
  • Integration of fluidic valves for precise flow regulation.
  • Dynamic interconnection and reconfiguration of chips in parallel, series, and complex arrangements.
  • Co-culture of intestinal epithelial cells and immune cells to model gut barrier function and inflammation.
  • Assessment of epithelial barrier integrity using transepithelial electrical resistance and permeability measurements.
  • Stimulation of immune cells and detection of inflammatory cytokine expression.

Main Results:

  • Successful development and demonstration of a modular microfluidic co-culture system.
  • Flexible chip arrangements facilitated dynamic cell-cell communication and spatial manipulation.
  • The system accurately modeled intestinal epithelial barrier function and inflammatory responses.
  • Transepithelial electrical resistance and permeability were modulated by dextran sulfate sodium treatment.
  • Inflammatory cytokine expression was detected upon immune cell stimulation through epithelial layers.

Conclusions:

  • The developed microfluidic system provides a versatile and modular platform for advanced cell co-culture.
  • It enables the study of complex cellular interactions and the impact of varying cell arrangements.
  • The system serves as a valuable tool for investigating disease mechanisms and screening potential therapeutic agents in a physiologically relevant context.