Related Experiment Video
Updated: May 24, 2025
11:24
Detection of Axonally Localized mRNAs in Brain Sections Using High-Resolution In Situ Hybridization
Published on: June 17, 2015
11.7K
Discovery of Novel Protein-Coding and Long Non-coding Transcripts in Distinct Regions of the Human Brain
Kristina Santucci1, Yuning Cheng1, Si-Mei Xu1
1School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, Australia.
Journal of Molecular Neuroscience : MN
|March 6, 2025
Summary
Researchers discovered 170 novel RNA isoforms, including messenger RNAs (mRNAs) and long non-coding RNAs (lncRNAs), in the human brain using advanced long-read sequencing. This enhances our understanding of brain transcriptomics.
Area of Science:
- Genomics
- Neuroscience
- Molecular Biology
Background:
- Long-read sequencing (LRS) technologies have advanced, improving accuracy in discovering and quantifying novel transcriptional isoforms.
- Accurate transcript quantification enables precise reconstruction of complex splicing patterns and transcriptomes.
Purpose of the Study:
- To leverage analytical developments in LRS for the discovery and analysis of RNA isoforms in the human brain.
- To identify and characterize novel RNA isoforms across different human brain tissues.
Main Methods:
- Utilized three bioinformatic tools to compile novel transcript isoforms.
- Quantified isoform expression across replicates of the cerebellar hemisphere, frontal cortex, and putamen.
- Curated a set of 170 highly confident novel RNA isoforms consistent across discovery methods.
Main Results:
- Identified 104 novel messenger RNA (mRNA) isoforms and 66 novel long non-coding RNA (lncRNA) isoforms.
- Detailed characterization of mRNA isoform BambuTx321, including structure, expression, and potential encoded proteins.
- Observed tissue-specific expression of lncRNA BambuTx1299 in the cerebellar hemisphere (mean CPM of 5.979).
Conclusions:
- Successfully identified and annotated numerous novel RNA isoforms in diverse human brain tissues.
- Provided insights into the expression patterns and potential functional roles of these novel isoforms.
- Contributed to a more comprehensive understanding of the brain's transcriptomic landscape for basic research applications.
Related Concept Videos
02:24Ribosome Profiling
3.4K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.4K
02:39lncRNA - Long Non-coding RNAs
8.4K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.4K