Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

IR and UV–Vis Spectroscopy of Aldehydes and Ketones01:29

IR and UV–Vis Spectroscopy of Aldehydes and Ketones

5.1K
Infrared spectroscopy, also known as vibrational spectroscopy, is mainly used to determine the types of bonds and functional groups in molecules. In aldehydes and ketones, the carbonyl (C=O) bond shows an absorption around 1710 cm-1. The C=O bond vibration of an aldehyde occurs at lower frequencies than that of a ketone. In addition to the C=O absorption in an aldehyde, the aldehydic C–H bond also gives two peaks in the 2700–2800 cm-1 range. This absorption, coupled with the...
5.1K
IR and UV–Vis Spectroscopy of Carboxylic Acids01:28

IR and UV–Vis Spectroscopy of Carboxylic Acids

4.6K
In IR spectroscopy of carboxylic acids, the C=O bond shows a characteristic band between 1710 and 1760 cm⁻¹, and the O–H bond exhibits a broad band between 2500 and 3300 cm⁻¹.
However, the stretching absorptions for the C=O bond vary depending on the structure of carboxylic acids. The C=O bond of the free carboxylic acids shows a higher stretching frequency, 1760 cm−1, while H-bonded carboxylic acids (dimers) exhibit stretching absorptions at a lower frequency,...
4.6K
UV–Vis Spectroscopy of Conjugated Systems01:32

UV–Vis Spectroscopy of Conjugated Systems

5.9K
Organic compounds with conjugated double bonds show strong absorption features in the UV–visible region of the electromagnetic spectrum attributed to π → π* electronic excitations. Generally, a UV–vis absorption spectrum is recorded as a plot of absorbance vs wavelength. The wavelength of maximum absorbance, which manifests as a peak in the absorption spectrum, is denoted as λmax.
One of the factors influencing λmax is...
5.9K
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

11.0K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
11.0K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.3K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.3K
Atomic Fluorescence Spectroscopy01:29

Atomic Fluorescence Spectroscopy

1.1K
Atomic fluorescence spectroscopy (AFS) is an analytical technique that involves the electronic transitions of atoms in a flame, furnace, or plasma being excited by electromagnetic (EM) radiation. When these atoms absorb energy, they become excited and subsequently release energy as they return to their original state. This emitted light, or "fluorescence," is observed at a right angle to the incident beam. Both absorption and emission processes transpire at distinct wavelengths, which...
1.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Built-In Self-Regeneration of Platinum Catalysis in Propane Dehydrogenation with Rare-Earth-Modified Zeolites.

Angewandte Chemie (International ed. in English)·2026
Same author

Molecular Probing in a Metal-Organic Framework for Selective Gas Adsorption and Separation.

Journal of the American Chemical Society·2026
Same author

Hexacoordinate Ti-Anchored Single-Atom Pd Catalyst for High-Efficiency Cyclohexanone Ammoximation with H<sub>2</sub> and O<sub>2</sub>.

Journal of the American Chemical Society·2026
Same author

In situ electron diffraction tomography study of the successive phase transitions in LaTaO<sub>4</sub> nanocrystals.

Nature communications·2026
Same author

Sequential ethane filling induced structural transitions in a flexible-robust metal-organic framework for ethylene purification.

Nature communications·2026
Same author

Artificial Intelligence Predicted OSDAs Enable Direct Synthesis of Interlayer-Expanded Zeolites.

Journal of the American Chemical Society·2026
Same journal

Efficient Chirality-Induced Spin Selectivity in Self-Assembled Monolayers of Ru<sub>2</sub><sup>5</sup><sup>+</sup> Paddlewheel Complexes.

Journal of the American Chemical Society·2026
Same journal

Direct Evidence for the Sulfonium-Mediated Photopolymerization of 1,2-Dithiolanes.

Journal of the American Chemical Society·2026
Same journal

Ionic Cluster Catalyst Assembly Strategy for Ethylene Polymerization and Copolymerization.

Journal of the American Chemical Society·2026
Same journal

Gate-Tailoring with Protons and Metal Cations in a Flexible Zeolite for High-Efficiency Ethylene/Ethane Separation.

Journal of the American Chemical Society·2026
Same journal

Pyridyl Radical-Induced Catalytic Reconstruction of Cyclic Sulfides.

Journal of the American Chemical Society·2026
Same journal

Probing Interfaces in Membrane Electrode Assemblies via <i>Operando</i> Infrared Spectroscopy at Model Gas-Liquid-Solid Triple-Phase Boundaries.

Journal of the American Chemical Society·2026
See all related articles

Related Experiment Video

Updated: May 3, 2026

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules
10:57

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules

Published on: November 2, 2009

12.8K

A Phototautomeric 3D Covalent Organic Framework for Ratiometric Fluorescence Humidity Sensing.

Xuan Yao1, Youchang Zhang1, Yu Qiu1

  • 1School of Physical Science and Technology, Shanghai Key Laboratory of High-Resolution Electron Microscopy, State Key Laboratory of Advanced Medical Materials and Devices, ShanghaiTech University, Shanghai 201210, China.

Journal of the American Chemical Society
|March 6, 2025
PubMed
Summary
This summary is machine-generated.

Researchers developed a new 3D covalent organic framework (COF) for sensitive humidity sensing. This phototautomeric material exhibits guest-induced fluorescence changes, enabling precise environmental monitoring.

More Related Videos

Qualitative Identification of Carboxylic Acids, Boronic Acids, and Amines Using Cruciform Fluorophores
09:46

Qualitative Identification of Carboxylic Acids, Boronic Acids, and Amines Using Cruciform Fluorophores

Published on: August 19, 2013

15.5K
Time-resolved Photophysical Characterization of Triplet-harvesting Organic Compounds at an Oxygen-free Environment Using an iCCD Camera
06:08

Time-resolved Photophysical Characterization of Triplet-harvesting Organic Compounds at an Oxygen-free Environment Using an iCCD Camera

Published on: December 27, 2018

8.8K

Related Experiment Videos

Last Updated: May 3, 2026

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules
10:57

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules

Published on: November 2, 2009

12.8K
Qualitative Identification of Carboxylic Acids, Boronic Acids, and Amines Using Cruciform Fluorophores
09:46

Qualitative Identification of Carboxylic Acids, Boronic Acids, and Amines Using Cruciform Fluorophores

Published on: August 19, 2013

15.5K
Time-resolved Photophysical Characterization of Triplet-harvesting Organic Compounds at an Oxygen-free Environment Using an iCCD Camera
06:08

Time-resolved Photophysical Characterization of Triplet-harvesting Organic Compounds at an Oxygen-free Environment Using an iCCD Camera

Published on: December 27, 2018

8.8K

Area of Science:

  • Photochemistry
  • Materials Science
  • Supramolecular Chemistry

Background:

  • Photoinduced proton transfer is crucial for advanced materials but challenging to control in condensed phases.
  • Excited State Intramolecular Proton Transfer (ESIPT) pathways require precise manipulation for functional applications.

Purpose of the Study:

  • To design a novel 3D covalent organic framework (COF) for efficient and controlled photoinduced proton transfer.
  • To develop a ratiometric fluorescence sensor for humidity detection with enhanced sensitivity and self-calibration.

Main Methods:

  • Integration of a hydronaphthoquinone fluorophore into a crystalline, porous, phototautomeric 3D COF.
  • Utilizing theoretical and spectroscopic studies to elucidate the ESIPT mechanism and tautomer dynamics.
  • Demonstrating humidity sensing capabilities through fluorescence turn-on, emission redshift, and lifetime shortening.

Main Results:

  • The 3D COF exhibits guest-induced fluorescence turn-on and emission redshift.
  • Shortened fluorescence lifetimes were observed, enabling ratiometric sensing.
  • The material demonstrated sensitive, rapid, steady, and self-calibrated humidity sensing across a wide range.

Conclusions:

  • The phototautomeric 3D COF provides a robust platform for ratiometric fluorescence humidity sensing.
  • The study offers molecular insights into designing functional porous materials for environmental and biomedical applications.
  • Integration of host-guest recognition and photoelectronic response is key for multiplexed sensing.