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Evidence for production of 1-acyl-2-acetyl-sn-glyceryl-3-phosphorylcholine concomitantly with platelet-activating
Biochemical and Biophysical Research Communications
|May 16, 1985
Abstract:
The presence of 1-acyl-2-acetyl-sn-glyceryl-3-phosphorylcholine in a sample of platelet-activating factor from stimulated rabbit neutrophils was demonstrated by a gas-liquid chromatography/mass spectrometry technique coupled with selected ion monitoring. The ions chosen for identification were those of acetyl and long-chain acyl moieties and molecular weight. Species containing palmitic, oleic and stearic acids were detected. A good correlation was observed between the productions of 1-acyl-2-acetyl-sn-glyceryl-3-phosphorylcholine and 1-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine by neutrophils stimulated with ionophore A23187.