Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Platelet-associated IgG is a specific protein.

M Steiner

    Biochemical and Biophysical Research Communications
    |May 31, 1985
    PubMed
    Summary
    This summary is machine-generated.

    Researchers isolated pure immunoglobulin G (IgG) that binds to human platelets using a novel cell column method. This technique effectively purified platelet-binding IgG from serum, yielding highly specific immunoglobulin preparations.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    [Decline of caries prevalence in Swiss military recruits between 1970 and 1996].

    Schweizer Monatsschrift fur Zahnmedizin = Revue mensuelle suisse d'odonto-stomatologie = Rivista mensile svizzera di odontologia e stomatologia·2001
    Same author

    The selective serotonin reuptake inhibitor sertraline: its profile and use in psychiatric disorders.

    CNS drug reviews·2001
    Same author

    The efficacy of fluoxetine in improving physical symptoms associated with premenstrual dysphoric disorder.

    BJOG : an international journal of obstetrics and gynaecology·2001
    Same author

    Serum-to-urinary prostate-specific antigen ratio in patients with benign prostatic hyperplasia and prostate cancer.

    Anticancer research·2001
    Same author

    Aged garlic extract, a modulator of cardiovascular risk factors: a dose-finding study on the effects of AGE on platelet functions.

    The Journal of nutrition·2001
    Same author

    Diagnosis and treatment of premenstrual dysphoric disorder: an update.

    International clinical psychopharmacology·2001
    Same journal

    The plasma membrane H<sup>+</sup>-ATPase OSA2 negatively regulates salt tolerance in rice seedlings.

    Biochemical and biophysical research communications·2026
    Same journal

    MiR-425-5p modulation of CREB1 affects inflammatory response and motor recovery after spinal cord injury.

    Biochemical and biophysical research communications·2026
    Same journal

    Xanthoangelol, a chalcone from Angelica keiskei, induces apoptosis in cervical cancer cell lines via the oxidative stress-mediated activation of caspase-8 and caspase-9.

    Biochemical and biophysical research communications·2026
    Same journal

    Suchilactone ameliorates podocyte injury in membranous nephropathy by modulating the JNK/p38 MAPK pathway in vitro.

    Biochemical and biophysical research communications·2026
    Same journal

    Palmitic acid-induced metabolic stress alters differentiation-associated gene expression in human ameloblast-like cells.

    Biochemical and biophysical research communications·2026
    Same journal

    A transferrin receptor-based vector enables robust Type-II membrane protein display on mammalian cells.

    Biochemical and biophysical research communications·2026
    See all related articles

    Area of Science:

    • Immunology
    • Biochemistry
    • Hematology

    Background:

    • Platelet-associated immunoglobulin G (PaIgG) plays a role in various immunological conditions.
    • Accurate isolation and characterization of PaIgG are crucial for understanding its function.
    • Previous methods for isolating PaIgG were often complex and yielded impure results.

    Purpose of the Study:

    • To develop a novel and efficient method for isolating immunologically pure immunoglobulin G (IgG) that binds specifically to human platelets.
    • To characterize the biochemical properties of the isolated platelet-binding IgG.
    • To assess the specificity and purity of the isolated IgG.

    Main Methods:

    • Development of a cell column technique using platelets or platelet membranes immobilized on an inert support via concanavalin A.

    Related Experiment Videos

  • Application of normal human IgG from pooled serum to the prepared cell columns.
  • Elution of bound IgG using a low pH buffer with high ionic strength.
  • Analysis of eluted IgG purity and molecular characteristics using isoelectric focusing on urea SDS polyacrylamide gels.

    • Main Results:

    • The cell column method successfully isolated immunologically and biochemically pure IgG that binds to human platelets.
    • Reapplication of non-adherent IgG to the column showed minimal further retention, indicating high specificity of the initial binding.
    • Isoelectric focusing revealed only two major bands (pIs 8.2 and 8.4) in the eluted IgG, contrasting with the broad pI range of the starting IgG.

    Conclusions:

    • The described cell column method provides an effective means for purifying platelet-specific immunoglobulin G (PaIgG).
    • This technique yields highly pure PaIgG, suitable for further immunological and biochemical investigations.
    • The characterized PaIgG exhibits a restricted heterogeneity, suggesting specific binding properties to human platelets.