Contaminant-driven midgut histological damage in bees and other aculeate Hymenoptera: A quantitative review

We present a review about histological sub-lethal effects due to anthropogenic contaminants on the midgut of bees and other aculeate hymenopterans. Contaminant types, damage types, and methodology were extracted and summarized from 74 published articles, and then quantitatively analyzed. We found that the Western honeybee (Apis mellifera) is by far the most widely used model. Contaminants have largely been tested under laboratory conditions, particularly insecticides and fungicides. Tissue-level damage (e.g., degradation of epithelium and of peritrophic membrane) were often detected together with cell-level damage (e.g., cell vacuolisation, karyorrhexis). Descriptive statistics and mixed models suggested that herbicides may cause a specific mix of alterations with an overall lower severity compared with other pesticides, while the combined use of light and electron microscopy seemed to detect more damage types. We claim for efforts to reduce biases in future studies on such histological effects, allowing their clearer use as markers of human activities.