Influence pathways of vanadium stress to microbial community in soil-tailings-groundwater systems

The large-scale exploitation of vanadium (V) bearing minerals has led to a massive accumulation of V tailings, of which V pollution poses severe ecological risks. Although the mechanisms of V stress to the microbial community have been reported, the influential pathways in a multi-medium-containing system, for example, the soil-tailings-groundwater system, are unknown. The dynamic redox conditions and substance exchange within the system exhibited complex V stress on the local microbial communities. In this study, the influence pathways of V stress to the microbial community in the soil-tailings-groundwater system were first investigated. High V contents were observed in groundwater (139.2 ± 0.15 µg/L) and soil (98.0-323.8 ± 0.02 mg/kg), respectively. Distinct microbial composition was observed for soil and groundwater, where soil showed the highest level of diversity and richness. Firmicutes, Proteobacteria, Actinobacteria, and Acidobacteria were dominant in soil and groundwater with a sum relative abundance of around 80 %. Based on redundancy analysis and structural equation models, V was one of the vital driving factors affecting microbial communities. Groundwater microbial communities were influenced by V via Cr, dissolved oxygen, and total nitrogen, while Fe, Mn, and total phosphorus were the key mediators for V to affect soil microbial communities. V affected the microbial community via metabolic pathways related to carbonaceous matter, which was involved in the establishment of survival strategies for metal stress. This study provides novel insights into the influence pathways of V on the microorganisms in tailings reservoir for pollution bioremediation.