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Enhancing Bacillus cereus antibacterial ability through improved cofactor supply.

Yinbiao Xu1,2,3, Jiasong Wu1,2,3, Tingting Yuan1,2,3

  • 1School of Life Sciences, Henan University, Kaifeng, 475004, China.

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This summary is machine-generated.

Bacillus cereus 0-9

Keywords:
Bacillus cereus 0–9Antimicrobial peptidesBiocontrol applicationGlucose dehydrogenaseSaturation mutagenesis

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Area of Science:

  • Microbiology
  • Biotechnology
  • Enzyme Engineering

Background:

  • Bacillus cereus 0-9 is a biocontrol agent with limited antibacterial activity.
  • Antimicrobial peptide secretion is key to its function.
  • Enhancing enzyme activity can improve biocontrol efficacy.

Purpose of the Study:

  • To improve the antibacterial activity of Bacillus cereus 0-9.
  • To enhance the catalytic efficiency of Bacillus subtilis glucose dehydrogenase (BsGDH).
  • To explore enzyme engineering strategies for biocontrol applications.

Main Methods:

  • Overexpression of Bacillus subtilis glucose dehydrogenase (BsGDH) in Bacillus cereus 0-9.
  • Site-directed mutagenesis of BsGDH based on conservation analysis and molecular docking.
  • Enzyme activity assays and kinetic analysis (Kcat/Km).
  • Molecular dynamics simulations to analyze conformational changes.

Main Results:

  • Overexpression of wild-type BsGDH increased antibacterial activity by 66%.
  • Mutant BsGDH (N97F/N192S/E198G) showed 5.66 times higher specific activity and 11.38 times higher Kcat/Km.
  • Engineered B. cereus 0-9 exhibited 2.26 times greater antibacterial activity.
  • Mutations enhanced active pocket flexibility and substrate affinity.

Conclusions:

  • Enzyme engineering of BsGDH significantly boosts the biocontrol potential of Bacillus cereus 0-9.
  • Targeted mutations improve enzyme catalytic efficiency and antibacterial output.
  • This approach offers a promising strategy for industrial biocontrol applications.