Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Antimicrobial photodynamic therapy reprograms resistance by inducing sensitization in methicillin-resistant Staphylococcus aureus.

Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology·2026
Same author

Evaluation of a time-resolved singlet oxygen detection system for in vivo photodynamic therapy.

Biomedical optics express·2026
Same author

Development of solid-state fluorescence lifetime standards for clinical applications using dyed epoxy resins.

Journal of biomedical optics·2026
Same author

A Membrane Fluidization Strategy Significantly Boosts Photodynamic Therapy and Antitumor Immunity of Porphyrin-Lipid Nanoparticles.

ACS nano medicine·2026
Same author

In Vivo Assessment of Benzoporphyrin Uptake and Singlet Oxygen Generation in Mice for Photodynamic Therapy Monitoring.

ACS photonics·2026
Same author

Photodynamic therapy dosimetry: current status and the emerging challenge of immune stimulation.

Journal of biomedical optics·2025

Related Experiment Video

Updated: May 24, 2025

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography
14:56

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography

Published on: May 20, 2022

3.7K

Atomically-precise Au22(Lys-Cys-Lys)16 nanoclusters for radiation sensitization.

Elham Zeinizade1,2, Goonay Yousefalizideh3, Parimah Aminfar3

  • 1Princess Margaret Cancer Centre, University Health Network, Toronto, ON, M5G 1L7, Canada.

Journal of Nanobiotechnology
|March 6, 2025
PubMed
Summary

Atomically precise gold nanoclusters, Au22(Lys-Cys-Lys)16, act as effective radiosensitizers, enhancing cancer radiotherapy by increasing tumor cell DNA damage and delaying tumor progression in vivo without observed side effects.

Keywords:
Atomically preciseBiocompatibilityCancer radiation therapyGold nanoclustersRadiosensitizerRenal clearanceUltra small size

More Related Videos

Author Spotlight: Direct Synthesis of EM-Visible Gold Nanoparticles in Cells for Protein Localization Analysis with Well-Preserved Ultrastructure
09:22

Author Spotlight: Direct Synthesis of EM-Visible Gold Nanoparticles in Cells for Protein Localization Analysis with Well-Preserved Ultrastructure

Published on: April 28, 2023

1.3K
Nanogold Labeling of the Yeast Endosomal System for Ultrastructural Analyses
09:49

Nanogold Labeling of the Yeast Endosomal System for Ultrastructural Analyses

Published on: July 14, 2014

10.2K

Related Experiment Videos

Last Updated: May 24, 2025

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography
14:56

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography

Published on: May 20, 2022

3.7K
Author Spotlight: Direct Synthesis of EM-Visible Gold Nanoparticles in Cells for Protein Localization Analysis with Well-Preserved Ultrastructure
09:22

Author Spotlight: Direct Synthesis of EM-Visible Gold Nanoparticles in Cells for Protein Localization Analysis with Well-Preserved Ultrastructure

Published on: April 28, 2023

1.3K
Nanogold Labeling of the Yeast Endosomal System for Ultrastructural Analyses
09:49

Nanogold Labeling of the Yeast Endosomal System for Ultrastructural Analyses

Published on: July 14, 2014

10.2K

Area of Science:

  • Nanomedicine
  • Biochemistry
  • Radiotherapy

Background:

  • Radiotherapy is crucial for cancer treatment but causes collateral damage to healthy tissues.
  • Radiosensitizers enhance radiotherapy efficacy while minimizing harm to normal cells.

Purpose of the Study:

  • To evaluate the radiosensitization potential, pharmacokinetics, biodistribution, and toxicity of Au22(Lys-Cys-Lys)16 nanoclusters.
  • To investigate the mechanisms underlying the radiosensitizing effects of these gold nanoclusters.

Main Methods:

  • Synthesis of atomically precise gold nanoclusters (Au22(Lys-Cys-Lys)16) via photochemical methods.
  • In vitro and in vivo evaluation of radiosensitization in various cancer cell lines and animal models.
  • Pharmacokinetic, biodistribution, and toxicity assessments.

Main Results:

  • Au22(Lys-Cys-Lys)16 nanoclusters demonstrated high stability, efficient tumor cell internalization, and significant dose enhancement factors (2.0 in KB, 1.6 in 4T1 cells).
  • Mechanistic studies revealed enhanced radiation-induced DNA damage and disrupted DNA repair pathways.
  • In vivo studies showed delayed tumor progression and prolonged survival in a KB tumor-bearing mouse model with no observed side effects.

Conclusions:

  • Au22(Lys-Cys-Lys)16 nanoclusters show significant radiosensitizing potential, offering a promising strategy for enhancing cancer radiotherapy.
  • These nanoclusters exhibit favorable pharmacokinetic properties and renal clearance, suggesting clinical translatability.