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Alterations in bile acid metabolites associated with pathogenicity and IVIG resistance in Kawasaki disease
Xinqi Wang1,2, Linli Han3, Jiyang Jiang1,2
1State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of Cariology and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
Insights
Kawasaki disease (KD) involves disturbed bile acid and lipid metabolism in children. Intravenous immunoglobulin (IVIG) treatment may help improve these metabolic changes, offering insights into KD
Area of Science:
- Metabolomics
- Biochemistry
- Pediatric Vasculitis
Background:
- Kawasaki disease (KD) is a critical pediatric condition characterized by acute systemic vasculitis.
- Metabolic disturbances are linked to cardiovascular disease risk in KD patients, but specific metabolic pathways remain unidentified.
Purpose of the Study:
- To investigate the specific metabolic alterations in children diagnosed with Kawasaki disease.
- To explore the impact of treatments like intravenous immunoglobulin (IVIG) and disease outcomes such as coronary artery occurrence on the metabolome of KD patients.
Main Methods:
- Utilized ultra-performance liquid chromatography (UPLC) and tandem mass spectrometry (MS) to analyze the metabolome.
- Compared the metabolomic profiles of 108 KD patients against 52 non-KD controls.
- Employed orthogonal projections to latent structures discriminant analysis (OPLS-DA) to assess group differences.
Main Results:
- Identified significantly disturbed bile acid and lipid production in KD patients compared to controls.
- Observed that IVIG treatment influences lipid and amino acid metabolism in KD.
- Found no significant metabolic differences between coronary injury and non-coronary injury groups; IVIG resistance did not appear to drive metabolic changes.
Conclusions:
- Kawasaki disease patients exhibit distinct metabolic abnormalities, particularly concerning bile acids and lipids.
- Intravenous immunoglobulin (IVIG) interventions show potential in mitigating some of the observed lipid abnormalities in KD.
Background:
Kawasaki disease (KD) primarily affects children as an acute systemic vasculitis. Numerous studies indicated an elevated risk of cardiovascular disease due to metabolic disturbances. Despite this knowledge, the specific metabolic modes involved in KD remain unclear.
Methods:
We examined the metabolome of individuals with 108 KD and 52 non-KD controls (KD vs. nKD) by ultraperformance liquid chromatography (UPLC) and tandem mass spectrometry (MS).
Results:
Differential analysis uncovered the disturbed production of bile acids and lipids in KD. Furthermore, we investigated the impact of treatment, intravenous immunoglobulin (IVIG) resistance, and coronary artery (CA) occurrence on the metabolome. Our findings suggested that IVIG treatment alters the lipid and amino acid metabolism of KD patients. By orthogonal projections to latent structures discriminant analysis (OPLS-DA), there was no significant difference between the coronary injury groups and non-coronary injury groups, and IVIG resistance didn't appear to cause the metabolic change in KD patients.
Conclusions:
Patients with KD exhibit metabolic abnormalities, particularly in bile acids and lipids. IVIG interventions may partially ameliorate these lipid abnormalities.
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