Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 23, 2025

Establishing Single-Cell Based Co-Cultures in a Deterministic Manner with a Microfluidic Chip
07:05

Establishing Single-Cell Based Co-Cultures in a Deterministic Manner with a Microfluidic Chip

Published on: September 27, 2019

6.0K

Hydrodynamic efficient cell capture and pairing method on microfluidic cell electrofusion chip.

Xuefeng Wang1, Yaqi Bai1, Xiaoling Zhang2

  • 1Key Laboratory of Biorheological Science and Technology, Ministry of Education and Bioengineering College, Chongqing University, Chongqing 400044, China.

APL Bioengineering
|March 7, 2025
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Integrated Microfluidic Chip Enabling Preparation and Immobilization of Cell-Laden Microspheres, and Microsphere-Based Cell Culture and Analysis.

Biosensors·2026
Same author

AI-Driven Smart Cockpit: Monitoring of Sudden Illnesses, Health Risk Intervention, and Future Prospects.

Sensors (Basel, Switzerland)·2026
Same author

Coarse-grained molecular dynamics simulation analysis of the effects of phospholipid and cholesterol on electroporation.

Artificial cells, nanomedicine, and biotechnology·2025
Same author

An Amplitude Analysis-Based Magnetoelastic Biosensing Method for Quantifying Blood Coagulation.

Biosensors·2025
Same author

Near-Infrared Optogenetic Nanosystem for Spatiotemporal Control of CRISPR-Cas9 Gene Editing and Synergistic Photodynamic Therapy.

ACS applied materials & interfaces·2024
Same author

Microfluidic Chip for Cell Fusion and In Situ Separation of Fused Cells.

Analytical chemistry·2024
Same journal

Shape factor analysis as a quantitative framework for assessing spheroid and organoid morphology and invasiveness.

APL bioengineering·2026
Same journal

HB-EGF enhances collective cell migration via spatial coordination of traction.

APL bioengineering·2026
Same journal

A pump-free microfluidic device for integrated multi-functional testing of tumor spheroids.

APL bioengineering·2026
Same journal

Photobiomodulation outperforms ultrasound in reducing IL-1 <b><i>β</i></b> -driven chondrocyte inflammation.

APL bioengineering·2026
Same journal

Research progress of 3D-printed anti-infective bone tissue engineering scaffolds based on triply periodic minimal surface structures.

APL bioengineering·2026
Same journal

Biomolecular and cellular chirality: Novel diagnostic perspectives for diseases.

APL bioengineering·2026
See all related articles

This study introduces a novel microfluidic device for efficient cell pairing, a key step in cell fusion for biotechnology. The device achieves high cell capture and pairing efficiencies, improving fusion yields.

Area of Science:

  • Biotechnology
  • Microfluidics
  • Cell Biology

Background:

  • Cell fusion is vital in biotechnology, but standard methods yield low efficiency due to poor cell contact.
  • Effective cell pairing is critical for successful cell fusion and improved yields.

Purpose of the Study:

  • To develop a novel microfluidic device for high-efficiency cell capture and pairing.
  • To optimize the device's performance through theoretical modeling and experimental validation.

Main Methods:

  • A microfluidic device employing a three-path symmetrical channel hydrodynamic capture method was designed.
  • Theoretical modeling and force analysis were used to understand cell trapping dynamics.
  • K562 cells were used to evaluate the effect of flow ratios on capture and pairing efficiency.

More Related Videos

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

14.8K
Cell Capture Using a Microfluidic Device
29:02

Cell Capture Using a Microfluidic Device

Published on: October 1, 2007

5.4K

Related Experiment Videos

Last Updated: May 23, 2025

Establishing Single-Cell Based Co-Cultures in a Deterministic Manner with a Microfluidic Chip
07:05

Establishing Single-Cell Based Co-Cultures in a Deterministic Manner with a Microfluidic Chip

Published on: September 27, 2019

6.0K
A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

14.8K
Cell Capture Using a Microfluidic Device
29:02

Cell Capture Using a Microfluidic Device

Published on: October 1, 2007

5.4K

Main Results:

  • The optimized device achieved a single-cell capture efficiency of approximately 95.6%.
  • A cell pairing efficiency of approximately 83.3% was obtained.
  • Subsequent electrofusion experiments on paired cells yielded a fusion efficiency of approximately 77.8%.

Conclusions:

  • The novel microfluidic device significantly enhances cell capture and pairing efficiency for cell fusion applications.
  • This technology offers a promising approach to improve yields in biotechnological processes relying on cell fusion.