[Effect of palmitate on energy coupling in lymphocyte mitochondria]

In the presence of 0.1 micrograms/ml of oligomycin, DNP (40-60 microM) increases lymphocyte respiration 10-fold and more. Palmitate taken at the same concentration stimulates the respiration of isolated mitochondria (1-2 mg prot/ml) in the presence of 1 mg/ml of BSA and the respiration of lymphocytes (10(8) cells/ml). When BSA and EGTA are absent in mitochondria isolation media, the mitochondrial respiration does not increase after DNP or ADP addition. Lymphocyte preparations are mostly distinguished by mitochondrial morphology in the presence of the uncoupler; they differ less by changes in dis-C3-(5) fluorescence after addition of 5-10 microM DNP and only insignificantly by the stimulation of respiration by DNP and palmitate. These results may be explained by the increase in the uncoupler-induced permeability of mitochondria for K+ and by partial transformation of delta psi m into delta pH in some cells, which may increase the cell resistance to damaging influences.