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Updated: May 23, 2025

A Robotic Platform for High-throughput Protoplast Isolation and Transformation
Published on: September 27, 2016
Protocol for generating protoplasts of Trichoderma koningiopsis and transformation of plasmid DNA
Sophie Jin1, Fabrizio Alberti1
1School of Life Sciences, University of Warwick, Gibbet Hill Road, Coventry CV4 7AL, UK.
Abstract:
Trichoderma koningiopsis is a filamentous fungus that produces numerous bioactive molecules and has the potential to be used as a biological control and plant growth-promoting agent. Here, we present a protocol for generating protoplasts of T. koningiopsis. We then describe four techniques for the stable transformation of plasmid DNA: polyethylene glycol (PEG)-mediated transformation of protoplasts, Agrobacterium-mediated transformation, electroporation of spores, and lipofection of mycelia. We then detail procedures for confirming the expression of a spCas9 gene using fluorescence microscopy.

