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Related Concept Videos

Spermatogenesis01:41

Spermatogenesis

Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male reproductive...

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Related Experiment Video

Updated: Jul 27, 2026

Evaluation of Intracellular Location of Reactive Oxygen Species in Solea Senegalensis Spermatozoa
10:24

Evaluation of Intracellular Location of Reactive Oxygen Species in Solea Senegalensis Spermatozoa

Published on: March 11, 2018

Increases in human sperm oxygen consumption at low cell concentrations.

D S Deutch, D F Katz, J W Overstreet

    Biology of Reproduction
    |May 1, 1985
    PubMed
    Summary

    Human sperm oxygen consumption increases as sperm concentration drops below 15-25 million cells/ml. This effect was observed in both natural semen and artificial media, with higher uptake in artificial settings.

    Area of Science:

    • Reproductive biology
    • Spermatozoa physiology
    • Cellular metabolism

    Background:

    • Understanding human sperm metabolism is crucial for reproductive health.
    • Spermatozoa oxygen consumption is a key indicator of cellular function.
    • Previous studies have explored factors influencing sperm metabolism, but concentration-dependent effects require further elucidation.

    Purpose of the Study:

    • To investigate the relationship between human spermatozoan concentration and oxygen consumption.
    • To compare oxygen uptake in natural semen versus an artificial medium.
    • To assess the stability of sperm oxygen consumption over short time intervals.

    Main Methods:

    • Human spermatozoa were serially diluted in autologous seminal plasma.
    • Washed spermatozoa were also diluted in an artificial medium.

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    High-Resolution Respirometry to Assess Mitochondrial Function in Human Spermatozoa
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    High-Resolution Respirometry to Assess Mitochondrial Function in Human Spermatozoa

    Published on: June 23, 2023

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    Last Updated: Jul 27, 2026

    Evaluation of Intracellular Location of Reactive Oxygen Species in Solea Senegalensis Spermatozoa
    10:24

    Evaluation of Intracellular Location of Reactive Oxygen Species in Solea Senegalensis Spermatozoa

    Published on: March 11, 2018

    Using an Extracellular Flux Analyzer to Measure Changes in Glycolysis and Oxidative Phosphorylation during Mouse Sperm Capacitation
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    Using an Extracellular Flux Analyzer to Measure Changes in Glycolysis and Oxidative Phosphorylation during Mouse Sperm Capacitation

    Published on: January 22, 2020

    High-Resolution Respirometry to Assess Mitochondrial Function in Human Spermatozoa
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    High-Resolution Respirometry to Assess Mitochondrial Function in Human Spermatozoa

    Published on: June 23, 2023

  • Oxygen consumption was measured using respirometry at varying sperm concentrations and over time.

    • Main Results:

    • Per-sperm oxygen uptake significantly increased when sperm concentration fell below 15-25 x 10^6 cells/ml in both media.
    • Oxygen consumption was consistently higher in the artificial medium compared to seminal plasma.
    • Sperm oxygen consumption in the artificial medium decreased over time, even without a significant loss of motility.

    Conclusions:

    • Human sperm oxygen consumption is concentration-dependent, with increased uptake at lower densities.
    • Artificial media can support higher rates of sperm oxygen consumption than seminal plasma.
    • Spermatozoa metabolism is dynamic and can decline even under conditions of maintained motility.